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Phospho-p38 MAPK (Thr180/Tyr182) (28B10) Mouse mAb (Sepharose® Bead Conjugate) #9219
Gallery: Phospho-p38 MAPK (Thr180/Tyr182) (28B10) Mouse mAb (Sepharose® Bead Conjugate) #9219
20 mM Tris-HCl (pH 7.4)
150 mM NaCl
1 mM Na2EDTA
1 mM EGTA
1% Triton X-100
2.5 mM sodium pyrophosphate
1 mM β-glycerophosphate
1 mM Na3VO4
1 µg/ml leupeptin
Note: Please aliquot prior to first use.Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol. Store at –20°C. Do not aliquot the antibodies.
Phospho-p38 MAPK (Thr180/Tyr182) Mouse mAb (Sepharose® Bead Conjugate) binds only p38 MAPK only when dually phosphorylated at Thr180 and Tyr182. This antibody was immobilized via conjugation of carbohydrates to cross-linked agarose hydrazide beads. This antibody does not significantly cross-react with phosphorylated SAPK/JNK or p44/42 MAPK, or with nonphosphorylated p38 MAPK.Species Reactivity: Human, Mouse, Rat, Monkey, S. cerevisiae Species predicted to react based on 100% sequence homology: Zebrafish
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Thr180/Tyr182 of human p38 MAP kinase.
p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAPK, p38α, β, γ (also known as Erk6 or SAPK3), and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors (1-5). MKK3, MKK6, and SEK activate p38 MAPK by phosphorylation at Thr180 and Tyr182. Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6), and MEF2 (5-8).
SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole) is a selective inhibitor of p38 MAPK. This compound inhibits the activation of MAPKAPK-2 by p38 MAPK and subsequent phosphorylation of HSP27 (9). SB203580 inhibits p38 MAPK catalytic activity by binding to the ATP-binding pocket, but does not inhibit phosphorylation of p38 MAPK by upstream kinases (10).
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For Research Use Only. Not For Use In Diagnostic Procedures. Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.