Revision 3

#33437Store at -20C

1 Kit

(9 x 20 microliters)

Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

Support: 877-678-TECH (8324)

Web: [email protected]

3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Product Includes Product # Quantity Mol. Wt Isotype/Source
MMP-1 (E9S9N) Rabbit mAb 54376 20 µl 55 kDa Rabbit IgG
MMP-2 (D4M2N) Rabbit mAb 40994 20 µl 64,72 kDa Rabbit IgG
MMP-9 (D6O3H) XP® Rabbit mAb 13667 20 µl 84, 92 kDa Rabbit IgG
MMP-13 (E4W3T) Rabbit mAb 69926 20 µl 60 kDa Rabbit IgG
Fibronectin/FN1 (E5H6X) Rabbit mAb 26836 20 µl 300 kDa Rabbit IgG
MMP-3 (D7F5B) Rabbit mAb 14351 20 µl 60 kDa Rabbit IgG
LOX (D8F2K) Rabbit mAb 58135 20 µl 54, 56 kDa Rabbit IgG
COL11A1 (E6O7R) Rabbit mAb 70458 20 µl 250 kDa Rabbit IgG
COL1A1 (E6A8E) Rabbit mAb 39952 20 µl 220 kDa Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl Goat 

Please visit for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.


The ECM Profiling Antibody Sampler Kit provides an economical means of detecting endogenous levels of the specific ECM components using the corresponding antibodies. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.


Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.


The extracellular matrix (ECM) is a cell surface associated three-dimensional macromolecular network composed of collagens, proteoglycans/glycosaminoglycans, elastin, fibronectin, laminins, and several other glycoproteins (1). The network provides a dynamic microenvironment surrounding the cell enabling it to carry on its function. Among the ECM proteins, fibronectin functions as a mediator to bridge distinct ECM components such as collagens, growth factors, as well as cell surface integrins to regulate ECM structural change and initiate signaling pathways (2). During normal development and pathological conditions, the ECM network is highly dynamic and continuously undergoes remodeling marked by the change of the ECM structural components, such as COL1A1, COL11A1, fibronectin, and versican. The matrix-degrading enzyme MMPs such as MMP1, MMP2, and MMP9 are highly involved in this process (4). Additional players in this process are the LOX family members of lysyl oxidase. They catalyze the first step of the covalent cross-linking of ECM proteins, collagens, and elastin, which contributes to ECM stiffness and mechanical properties (5).

  1. Theocharis, A.D. et al. (2019) FEBS J 286, 2830-69.
  2. Zollinger, A.J. and Smith, M.L. (2017) Matrix Biol 60-1, 27-37.
  3. Paolillo, M. and Schinelli, S. (2019) Int J Mol Sci 20, pii: E4947. doi: 10.3390/ijms20194947.
  4. Bonnans, C. et al. (2014) Nat Rev Mol Cell Biol 15, 786-801.
  5. Amendola, P.G. et al. (2019) Cancers (Basel) 11, pii: E729. doi: 10.3390/cancers11050729.

Background References

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