Revision 1

#48267Store at -20C

1 Kit

(8 x 20 microliters)

Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

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3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Product Includes Product # Quantity Mol. Wt Isotype/Source
mGluR1 (D5H10) Rabbit mAb 12551 20 µl 145, >300 kDa Rabbit IgG
mGluR5 (D6E7B) Rabbit mAb 55920 20 µl 150, 300 kDa Rabbit IgG
FMRP (D14F4) Rabbit mAb 7104 20 µl 80 kDa Rabbit IgG
FXR1 (D10A2) XP® Rabbit mAb 12295 20 µl 78-80, 82-84 kDa Rabbit IgG
FXR2 (D85D6) Rabbit mAb 7098 20 µl 95 kDa Rabbit IgG
CYFIP1 Antibody 44353 20 µl 145 kDa Rabbit 
Phospho-eEF2 (Thr56) Antibody 2331 20 µl 95 kDa Rabbit 
eEF2 Antibody 2332 20 µl 95 kDa Rabbit 
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl Goat 

Please visit for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.


The Fragile X/FMRP Signaling Pathway Antibody Sampler Kit provides an economical means of detecting signaling components of the Fragile X/FMRP signaling pathway. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.


Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibodies.


Fragile X syndrome, a frequent cause of inherited mental retardation, often results from expansion of the CGG trinucleotide repeat in the gene that encodes the fragile X mental retardation protein (FMRP, [1]). FMRP (also known as FMR1) and its two autosomal homologs (FXR1 and FXR2) all bind RNA and play a role in the pathogenesis of fragile X syndrome (1-3). Each of these related proteins can associate with one another as well as form homodimers and complexes with other RNA-binding proteins like cytoplasmic FMRP interacting protein 1 (CYFIP1, [3,4]). FMRP, FXR1, FXR2, and CYFIP1 have been implicated in the translational regulation of mRNAs (5,6). Importantly, this complex of proteins may be dynamically regulated to drive protein synthesis-dependent forms of synaptic plasticity in response to specific activity. That is, activation of metabotropic glutamate receptors, including mGluR1 and mGlur5, can regulate FMRP-dependent forms of translation via post-translational modification of eukaryotic elongation factor 2 (eEF2) to locally control dynamic translation of important synaptic proteins, which, subsequently, alter synaptic function (7-9).   

  1. Verkerk, A.J. et al. (1991) Cell 65, 905-14.
  2. Siomi, M.C. et al. (1995) EMBO J 14, 2401-8.
  3. Zhang, Y. et al. (1995) EMBO J 14, 5358-66.
  4. Abekhoukh, S. et al. (2017) Dis Model Mech 10, 463-74.
  5. Linder, B. et al. (2008) Hum Mol Genet 17, 3236-46.
  6. De Rubeis, S. et al. (2013) Neuron 79, 1169-82.
  7. Park, S. et al. (2008) Neuron 59, 70-83.
  8. Barnes, S.A. et al. (2015) J Neurosci 35, 15073-81.
  9. Paul, A. et al. (2019) Front Mol Neurosci 12, 97.

Background References

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