Revision 3

#52239Store at -20C

1 Kit

(9 x 20 microliters)

Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

Support: 877-678-TECH (8324)

Web: [email protected]

3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Product Includes Product # Quantity Mol. Wt Isotype/Source
Phospho-STING (Ser366) (D7C3S) Rabbit mAb 19781 20 µl 40 kDa Rabbit IgG
STING (D2P2F) Rabbit mAb 13647 20 µl 33, 35 kDa Rabbit IgG
Phospho-IRF-3 (Ser396) (D6O1M) Rabbit mAb 29047 20 µl 45-55 kDa Rabbit IgG
IRF-3 (D6I4C) XP® Rabbit mAb 11904 20 µl 50-55 kDa Rabbit IgG
Phospho-IRAK4 (Thr345/Ser346) (D6D7) Rabbit mAb 11927 20 µl 55 kDa Rabbit IgG
IRAK4 Antibody 4363 20 µl 55 kDa Rabbit 
Phospho-IRF-7 (Ser477) (D7E1W) Rabbit mAb 12390 20 µl 65 kDa Rabbit IgG
IRF-7 (D2A1J) Rabbit mAb 13014 20 µl 65 kDa Rabbit IgG
Cleaved-IL-1β (Asp116) (D3A3Z) Rabbit mAb 83186 20 µl 17 kDa Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl Goat 

Please visit for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.


The Innate Immunity Activation Antibody Sampler Kit provides an economical means of detecting the activation of multiple signaling pathways involved in innate immunity using phospho-specific, cleavage-specific, and control antibodies. The kit contains enough primary antibodies to perform at least two western blot experiments.


Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibodies.


The innate immune system responds rapidly to pathogens by detecting conserved pathogen-associated molecular patterns (PAMPs) and damage/danger-associated molecular patterns (DAMPs) through pattern recognition receptors (PRRs). There are several families of PRRs. Toll-like receptors (TLRs) are transmembrane PRRs and signal through recruitment of adaptor proteins, including MyD88, which leads to recruitment and phosphorylation of IRAK1 and IRAK4, followed by activation of NF-κB and MAP kinases (1-3). Some TLRs also activate IRFs, which upregulate the type I interferon response. Activation of TLR3 and TLR4 results in phosphorylation and activation of IRF-3, while TLR7, TLR8, and TLR9 lead to activation of IRF-7 (2, 3). STING is a multi-pass ER transmembrane protein that is activated in response to intracellular DNA downstream of DNA-sensing cytoplasmic PRRs, such as DDX41, or by binding the second messenger cyclic-GMP-AMP (cGAMP) produced by cGAS (4-6). Following activation, STING translocates with TBK1 to perinuclear endosomes, leading to phosphorylation and activation of IRF-3 and NF-κB (7, 8). Following activation and translocation, STING gets phosphorylated by ULK1, resulting in STING inactivation and degradation (9). Inflammasomes are cytoplasmic multimeric protein complexes that assemble in response to PAMPs or DAMPs detected by AIM2 or members of the nod-like receptor (NLR) family, such as NLRP3 (10). Inflammasomes activate Caspase-1, which cleaves the IL-1β and IL-18 precursor proteins into the mature forms (10).

  1. Janssens, S. and Beyaert, R. (2003) Mol Cell 11, 293-302.
  2. Barton, G.M. and Kagan, J.C. (2009) Nat Rev Immunol 9, 535-42.
  3. Blasius, A.L. and Beutler, B. (2010) Immunity 32, 305-15.
  4. Ishikawa, H. and Barber, G.N. (2008) Nature 455, 674-8.
  5. Zhang, Z. et al. (2011) Nat Immunol 12, 959-65.
  6. Sun, L. et al. (2013) Science 339, 786-91.
  7. Zhong, B. et al. (2008) Immunity 29, 538-50.
  8. Ishikawa, H. et al. (2009) Nature 461, 788-92.
  9. Konno, H. et al. (2013) Cell 155, 688-98.
  10. Rathinam, V.A. and Fitzgerald, K.A. (2016) Cell 165, 792-800.

Background References

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