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PhosphoSitePlus® Resource

  • Additional protein information
  • Analytical tools

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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Phospho-STING (Ser366) Antibody 85735 20 µl
Western Blotting Immunoprecipitation
H 40, 48, 50 Rabbit 
STING (D2P2F) Rabbit mAb 13647 20 µl
Western Blotting Immunoprecipitation Immunohistochemistry
H M 33, 35 Rabbit IgG
Phospho-IRF-3 (Ser396) (D6O1M) Rabbit mAb 29047 20 µl
Western Blotting Immunoprecipitation Immunofluorescence Flow Cytometry
H M R 45-55 Rabbit IgG
IRF-3 (D6I4C) XP® Rabbit mAb 11904 20 µl
Western Blotting Immunoprecipitation Immunofluorescence
H Mk 50-55 Rabbit IgG
Phospho-IRAK4 (Thr345/Ser346) (D6D7) Rabbit mAb 11927 20 µl
Western Blotting Immunoprecipitation Flow Cytometry
H 55 Rabbit IgG
IRAK4 Antibody 4363 20 µl
Western Blotting Immunoprecipitation
H M R Mk 55 Rabbit 
Phospho-IRF-7 (Ser477) (D7E1W) Rabbit mAb 12390 20 µl
Western Blotting
H 65 Rabbit IgG
IRF-7 (D2A1J) Rabbit mAb 13014 20 µl
Western Blotting
H 65 Rabbit IgG
Cleaved-IL-1β (Asp116) (D3A3Z) Rabbit mAb 83186 20 µl
Western Blotting Immunoprecipitation Immunofluorescence
H 17 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
Western Blotting
Goat 

Product Description

The Innate Immunity Activation Antibody Sampler Kit provides an economical means of detecting the activation of multiple signaling pathways involved in innate immunity using phospho-specific, cleavage-specific, and control antibodies. The kit contains enough primary antibodies to perform at least two western blot experiments.


Specificity / Sensitivity

Each antibody in the Innate Immunity Activation Antibody Sampler Kit detects endogenous levels of its target protein. Phospho-STING (Ser366) Antibody detects STING only when phosphorylated at Ser366. Phospho-IRF-3 (Ser396) (D6O1M) Rabbit mAb detects IRF-3 only when phosphorylated at Ser396. Phospho-IRAK4 (Thr345/Ser346) (D6D7) Rabbit mAb detects IRAK4 only when phosphorylated at both Thr345 and Ser346 and does not react with IRAK4 when phosphorylated at only one of these sites. Phospho-IRF-7 (Ser477) (D7E1W) Rabbit mAb detects IRF-7 only when phosphorylated at Ser477. Phospho-IRF-7 (Ser477) (D7E1W) Rabbit mAb may cross-react with proteins of unknown origin between 100 and 150 kDa. Cleaved-IL-1β (Asp116) (D3A3Z) Rabbit mAb detects mature IL-1β only when cleaved at Asp116.


Source / Purification

Monoclonal and polyclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Pro226 of STING, Lys41 of IRAK4, Pro115 of IRF-7, Asp116 of IL-1β, or recombinant human IRF-3 protein. Phospho-specific antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Ser366 of STING, Ser396 of IRF-3, Thr345/Ser346 of IRAK4, or Ser477 of IRF-7. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

The innate immune system responds rapidly to pathogens by detecting conserved pathogen-associated molecular patterns (PAMPs) and damage/danger-associated molecular patterns (DAMPs) through pattern recognition receptors (PRRs). There are several families of PRRs. Toll-like receptors (TLRs) are transmembrane PRRs and signal through recruitment of adaptor proteins, including MyD88, which leads to recruitment and phosphorylation of IRAK1 and IRAK4, followed by activation of NF-κB and MAP kinases (1-3). Some TLRs also activate IRFs, which upregulate the type I interferon response. Activation of TLR3 and TLR4 results in phosphorylation and activation of IRF-3, while TLR7, TLR8, and TLR9 lead to activation of IRF-7 (2, 3). STING is a multi-pass ER transmembrane protein that is activated in response to intracellular DNA downstream of DNA-sensing cytoplasmic PRRs, such as DDX41, or by binding the second messenger cyclic-GMP-AMP (cGAMP) produced by cGAS (4-6). Following activation, STING translocates with TBK1 to perinuclear endosomes, leading to phosphorylation and activation of IRF-3 and NF-κB (7, 8). Following activation and translocation, STING gets phosphorylated by ULK1, resulting in STING inactivation and degradation (9). Inflammasomes are cytoplasmic multimeric protein complexes that assemble in response to PAMPs or DAMPs detected by AIM2 or members of the nod-like receptor (NLR) family, such as NLRP3 (10). Inflammasomes activate Caspase-1, which cleaves the IL-1β and IL-18 precursor proteins into the mature forms (10).


1.  Janssens, S. and Beyaert, R. (2003) Mol Cell 11, 293-302.

2.  Barton, G.M. and Kagan, J.C. (2009) Nat Rev Immunol 9, 535-42.

3.  Blasius, A.L. and Beutler, B. (2010) Immunity 32, 305-15.

4.  Ishikawa, H. and Barber, G.N. (2008) Nature 455, 674-8.

5.  Zhang, Z. et al. (2011) Nat Immunol 12, 959-65.

6.  Sun, L. et al. (2013) Science 339, 786-91.

7.  Zhong, B. et al. (2008) Immunity 29, 538-50.

8.  Ishikawa, H. et al. (2009) Nature 461, 788-92.

9.  Konno, H. et al. (2013) Cell 155, 688-98.

10.  Rathinam, V.A. and Fitzgerald, K.A. (2016) Cell 165, 792-800.


Entrez-Gene Id 3553 , 51135 , 3661 , 3665 , 340061
Swiss-Prot Acc. P01584 , Q9NWZ3 , Q14653 , Q92985 , Q86WV6


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
Tween is a registered trademark of ICI Americas, Inc.

52239
Innate Immunity Activation Antibody Sampler Kit