| Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
|---|---|---|---|---|
| RNase L (D4B4J) Rabbit mAb | 27281 | 20 µl | 80 kDa | Rabbit IgG |
| OAS1 (D1W3A) Rabbit mAb | 14498 | 20 µl | 40, 44 kDa | Rabbit IgG |
| OAS2 (E2G4K) Rabbit mAb | 24344 | 20 µl | 70 kDa | Rabbit IgG |
| OAS3 (E4X7Z) Rabbit mAb | 74906 | 20 µl | 120 kDa | Rabbit IgG |
| OASL (E7W1R) Rabbit mAb | 36845 | 20 µl | 52 kDa | Rabbit IgG |
| PDE12 (E2V7J) Rabbit mAb | 88499 | 20 µl | 75, 70 kDa | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat |
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Description
The OAS/RNase L Pathway Antibody Sampler Kit provides an economical means of detecting proteins involved in the OAS/RNase L pathway. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.
Storage
Background
The 2'-5'-oligoadenylate synthetase (OAS)/RNase L pathway is a component of the antiviral innate immune system. The OAS family of proteins includes OAS1, OAS2, OAS3, and OASL in humans, all of which are induced in response to type 1 interferons (1-3). When activated by cytosolic double-stranded RNA, OAS1-3 generate 2’-5’-linked oligoadenylates that bind to and activate the ribonuclease, RNase L. Activated RNase L is poised to degrade viral and cellular RNA as part of the antiviral innate immune response (4). Unlike OAS1-3, OASL lacks intrinsic enzymatic activity, and research studies have suggested that OASL participates in the antiviral immune response independently of the canonical OAS/RNase L signaling pathway (5,6). Negative regulation of the OAS/RNase L pathway is governed, in part, by the PDE12 phosphodiesterase that degrades 2'-5'-oligoadenylates (7).
- Chebath, J. et al. Nature 330, 587-8.
- Schoggins, J.W. et al. (2011) Nature 472, 481-5.
- (2019) Proc Natl Acad Sci U S A 116, 12573.
- Dong, B. and Silverman, R.H. (1997) J Biol Chem 272, 22236-42.
- Marques, J. et al. (2008) J Gen Virol 89, 2767-2772.
- Ishibashi, M. et al. (2010) Biochem Biophys Res Commun 392, 397-402.
- Wood, E.R. et al. (2015) J Biol Chem 290, 19681-96.
Background References
Trademarks and Patents
Limited Uses
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