Revision 1

#74798Store at -20C

1 Kit

(8 x 20 microliters)

Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

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3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Product Includes Product # Quantity Mol. Wt Isotype/Source
PSD95 (D74D3) XP® Rabbit mAb 3409 20 µl 95 kDa Rabbit IgG
Synaptophysin (D8F6H) XP® Rabbit mAb 36406 20 µl 38 kDa Rabbit IgG
Bassoon (D63B6) Rabbit mAb 6897 20 µl 420 kDa Rabbit IgG
Complexin-1/2 (D8A6E) Rabbit mAb 28070 20 µl 14-16 kDa Rabbit IgG
Synaptotagmin-1 (D33B7) Rabbit mAb 14558 20 µl 60 kDa Rabbit IgG
SHANK3 (D5K6R) Rabbit mAb 64555 20 µl 220 kDa Rabbit IgG
Synapsin-1 (D12G5) XP® Rabbit mAb 5297 20 µl 77 kDa Rabbit IgG
SynGAP (D78B11) Rabbit mAb 5540 20 µl 140 kDa Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl Goat 

Please visit for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.


The Synaptic Neuron Marker Antibody Sampler Kit provides an economical means of detecting presynaptic and postsynaptic proteins by western blot. This kit includes enough primary antibodies to perform at least two western blot experiments with each primary antibody.


Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.


Synaptophysin (SYP) is a neuronal synaptic vesicle glycoprotein that is expressed in neuroendocrine cells and neoplasms (1). Synapsin-1 is a neuronal phospho-protein localized to presynaptic terminals. Synapsin-1 plays an important role in synapse formation, neurotransmitter regulation, and regulation of synaptic vesicle fusion and trafficking (2,3). Synaptotagmin-1 (SYT1) is an integral membrane protein found in synaptic vesicles thought to play a role in vesicle trafficking and exocytosis (4). Complexin isoforms 1 and 2 are small synaptic proteins that bind to SNARE complexes, responsible for regulating exocytosis and synaptic vesicle fusion (5). SynGAP is a synaptic GTPase-activating protein selectively expressed in the brain and found at higher concentrations specifically at excitatory synapses in the mammalian forebrain. SynGAP interacts with the PDZ domains of PSD95, a postsynaptic scaffolding protein that couples SynGAP to NMDA receptors (6). PSD95 is involved in the assembly and function of the postsynaptic density (PSD) complex (7,8). SHANK proteins act as scaffolds at the neuronal PSD (9), where they play a critical role in PSD assembly of excitatory synapses during development (10). Bassoon (BSN) is a scaffolding protein component of the synaptic ribbon and of the cytomatrix at the active zones of both excitatory and inhibitory synapses with a presumptive role in orchestrating events of the synaptic vesicle cycle (11-13). Together, these proteins can be used to measure presynaptic and postsynaptic proteins and synaptic development under normal and disease conditions.

  1. Wiedenmann, B. et al. (1986) Proc Natl Acad Sci U S A 83, 3500-4.
  2. Mirza, F.J. and Zahid, S. (2018) Neurosci Bull 34, 349-358.
  3. Takei, Y. et al. (1995) J Cell Biol 131, 1789-800.
  4. Fukuda, M. and Mikoshiba, K. (2001) Biochem Biophys Res Commun 281, 1226-33.
  5. Chang, S. et al. (2015) J Neurosci 35, 8272-90.
  6. Kim, J.H. et al. (1998) Neuron 20, 683-91.
  7. Cao, J. et al. (2005) J Cell Biol 168, 117-26.
  8. Chetkovich, D.M. et al. (2002) J Neurosci 22, 6415-25.
  9. Grabrucker, A.M. et al. (2011) Trends Cell Biol 21, 594-603.
  10. Boeckers, T.M. et al. (1999) J Neurosci 19, 6506-18.
  11. Winter, C. et al. (1999) Genomics 57, 389-97.
  12. Hallermann, S. et al. (2010) Neuron 68, 710-23.
  13. Frank, T. et al. (2010) Neuron 68, 724-38.

Background References

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