Synaptic Neuron Marker Antibody Sampler Kit #74798
Product Information
Kit Usage Information
Protocols
- 3409: Western Blotting, Immunohistochemistry (Paraffin)
- 5297: Western Blotting, Immunoprecipitation (Magnetic), Immunohistochemistry (Paraffin), Immunofluorescence
- 5540: Western Blotting, Immunoprecipitation (Magnetic)
- 6897: Western Blotting, Immunofluorescence
- 7074: Western Blotting
- 14558: Western Blotting, Immunoprecipitation (Magnetic)
- 28070: Western Blotting, Immunoprecipitation (Agarose), Immunofluorescence*
- 36406: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence
- 64555: Western Blotting, Immunoprecipitation (Agarose), IF-F Citrate Retrieval (Rabbit)
Product Description
Specificity / Sensitivity
Each antibody in the Synaptic Neuron Marker Antibody Sampler Kit detects endogenous levels of its target protein. Synapsin-1 (D12G5) XP® Rabbit mAb detects endogenous levels of total synapsin protein. The antigen is 100% conserved between human synapsin-1a and synapsin-1b. Bassoon (D63B6) Rabbit mAb recognizes endogenous levels of total bassoon protein. Some background staining was observed in muscle tissue and the choroid plexus by confocal immunofluorescent analysis. Synaptotagmin-1 (D33B7) Rabbit mAb recognizes endogenous levels of total synaptotagmin-1 protein. This antibody may also cross-react with an unidentified protein of approximately 45 kDa.
Source / Purification
Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly299 of human Synaptophysin protein, Gln1217 of human bassoon protein, Pro125 of human complexin-2 protein, Arg400 of human synaptotagmin-1 protein, Ile1210 of human SHANK3 protein, Gln483 of human synapsin-1 protein, Arg732 of human SynGAP protein, and the amino-terminal of human PSD95 protein.
Background
Synaptophysin (SYP) is a neuronal synaptic vesicle glycoprotein that is expressed in neuroendocrine cells and neoplasms (1). Synapsin-1 is a neuronal phospho-protein localized to presynaptic terminals. Synapsin-1 plays an important role in synapse formation, neurotransmitter regulation, and regulation of synaptic vesicle fusion and trafficking (2,3). Synaptotagmin-1 (SYT1) is an integral membrane protein found in synaptic vesicles thought to play a role in vesicle trafficking and exocytosis (4). Complexin isoforms 1 and 2 are small synaptic proteins that bind to SNARE complexes, responsible for regulating exocytosis and synaptic vesicle fusion (5). SynGAP is a synaptic GTPase-activating protein selectively expressed in the brain and found at higher concentrations specifically at excitatory synapses in the mammalian forebrain. SynGAP interacts with the PDZ domains of PSD95, a postsynaptic scaffolding protein that couples SynGAP to NMDA receptors (6). PSD95 is involved in the assembly and function of the postsynaptic density (PSD) complex (7,8). SHANK proteins act as scaffolds at the neuronal PSD (9), where they play a critical role in PSD assembly of excitatory synapses during development (10). Bassoon (BSN) is a scaffolding protein component of the synaptic ribbon and of the cytomatrix at the active zones of both excitatory and inhibitory synapses with a presumptive role in orchestrating events of the synaptic vesicle cycle (11-13). Together, these proteins can be used to measure presynaptic and postsynaptic proteins and synaptic development under normal and disease conditions.
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- Mirza, F.J. and Zahid, S. (2018) Neurosci Bull 34, 349-358.
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- Chang, S. et al. (2015) J Neurosci 35, 8272-90.
- Kim, J.H. et al. (1998) Neuron 20, 683-91.
- Cao, J. et al. (2005) J Cell Biol 168, 117-26.
- Chetkovich, D.M. et al. (2002) J Neurosci 22, 6415-25.
- Grabrucker, A.M. et al. (2011) Trends Cell Biol 21, 594-603.
- Boeckers, T.M. et al. (1999) J Neurosci 19, 6506-18.
- Winter, C. et al. (1999) Genomics 57, 389-97.
- Hallermann, S. et al. (2010) Neuron 68, 710-23.
- Frank, T. et al. (2010) Neuron 68, 724-38.
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