Revision 1

#85314Store at -20C

1 Kit

(7 x 20 microliters)

Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

Support: 877-678-TECH (8324)

Web: [email protected]

3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Product Includes Product # Quantity Mol. Wt Isotype/Source
APP Antibody 2452 20 µl 100 to 140 kDa Rabbit 
β-Amyloid (1-42) (D3E10) Rabbit mAb 12843 20 µl 4 kDa Rabbit 
β-Amyloid (1-40) (D8Q7I) Rabbit mAb 12990 20 µl 4 kDa Rabbit IgG
β-Amyloid (1-37) (D2A6H) Rabbit mAb 12467 20 µl 4 kDa Rabbit IgG
β-Amyloid (1-39) (D5Y9L) Rabbit mAb 12077 20 µl 4 kDa Rabbit IgG
β-Amyloid (D54D2) XP® Rabbit mAb 8243 20 µl 5 kDa Rabbit IgG
β-Amyloid (pE3 Peptide) (D5N5H) Rabbit mAb 14975 20 µl 4 kDa Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl Goat 

Please visit for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.


The β-Amyloid Antibody Sampler Kit provides an economical means of detecting APP and APP unmodified/modified fragments using total and fragment-specific antibodies. The kit includes enough antibody to perform two western blot experiments with each primary antibody.


Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.


Amyloid β (Aβ) precursor protein (APP) is a 100-140 kDa transmembrane glycoprotein that exists as several isoforms (1). The amino acid sequence of APP contains an amyloid domain, which can be processed and released by two-step proteolytic cleavage (1). The extracellular deposition and accumulation of the released Aβ fragments form the main components of amyloid plaques in Alzheimer's disease (1). Several fragments corresponding to progressive APP processing at alternative cleavage sites have been identified (2). These include Aβ (1-37), Aβ (1-39), Aβ (1-40), and Aβ (1-42) (2). These fragments can also be N-terminally modified to generate pyroglutamate-3 Aβ (pE3-peptide) (3). Fragment-specific and pan-Aβ antibodies are used to detect and examine relative levels of individual Aβ fragments.

  1. Selkoe, D.J. (1996) J Biol Chem 271, 18295-8.
  2. Selkoe, D.J. and Hardy, J. (2016) EMBO Mol Med 8, 595-608.
  3. Saido, T.C. et al. (1995) Neuron 14, 457-66.

Background References

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