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85314
β-Amyloid Antibody Sampler Kit

β-Amyloid Antibody Sampler Kit #85314

Western Blotting Image 1

Western blot analysis of extracts from HeLa (human), 3T3L1 (mouse) or C6 (rat) cells, untreated or nocodazole-treated (1 µg/ml, 16-18 hours), using APP Antibody.

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Western Blotting Image 2

Western blot analysis of the indicated amounts of human Aβ-42 peptide using β-Amyloid (1-42 Specific) (D3E10) Rabbit mAb.

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Western Blotting Image 3

Western blot analysis of human Aβ-37, Aβ-38, Aβ-39, Aβ-40, Aβ-42, and Aβ-43 peptides (2.5 ng) using β-Amyloid (1-42 Specific) (D3E10) Rabbit mAb (upper) or β-Amyloid (D54D2) XP® Rabbit mAb #8243 (lower).

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Western Blotting Image 4

Western blot analysis of brain extracts from 13-month old wild-type and TG2576 mice using β-Amyloid (1-40 Specific) (D8Q7I) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

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Western Blotting Image 5

Western blot analysis of the indicated amounts of human Aβ-37 peptide using β-Amyloid (1-37 Specific) (D2A6H) Rabbit mAb.

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Western Blotting Image 6

Western blot analysis of the indicated amounts of human Aβ-39 peptide using β-Amyloid (1-39 Specific) (D5Y9L) Rabbit mAb.

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Western Blotting Image 7

Western blot analysis of human Aβ-42, Aβ-40, Aβ-39, Aβ-38, and Aβ-37 peptides (5 ng) using β-Amyloid (D54D2) XP® Rabbit mAb.

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Western Blotting Image 8

β-Amyloid (pE3 Peptide) (D5N5H) Rabbit mAb specificity is demonstrated using peptide dot blot. β-Amyloid (pE3 Peptide) (D5N5H) Rabbit mAb binds to pre-coated β-amyloid (pE3) peptide but not to β-amyloid (E3) peptide or scrambled β-amyloid (pE3) peptide.

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Western Blotting Image 9

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

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IF-IC Image 10

Confocal microscopic images of SH-SY5Y cells showing cytoplasmic and ER stain with APP Antibody (A, red) compared to an isotype control (B). Blue pseudocolor = DRAQ5ä (fluorescent DNA dye).

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Western Blotting Image 11

Western blot analysis of human Aβ-37, Aβ-38, Aβ-39, Aβ-40, Aβ-42 and Aβ-43 peptides (10 ng) using β-Amyloid (1-40 Specific) (D8Q7I) Rabbit mAb (upper) and β-Amyloid (D54D2) XP® Rabbit mAb #8243 (lower).

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Western Blotting Image 12

Western blot analysis of brain extracts from 13-month old wild-type and TG2576 mice using β-Amyloid (1-37 Specific) (D2A6H) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

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Western Blotting Image 13

Western blot analysis of human Aβ-37, Aβ-38, Aβ-39, Aβ-40, Aβ-42, and Aβ-43 peptides (10 ng) using β-Amyloid (1-39 Specific) (D5Y9L) Rabbit mAb (upper) or β-Amyloid (D54D2) XP® Rabbit mAb #8243 (lower).

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Western Blotting Image 14

Western blot analysis of the indicated amounts of human Aβ-42 (left) and Aβ-40 (right) peptides using β-Amyloid (D54D2) XP® Rabbit mAb.

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Western Blotting Image 15

Western blot analysis of the indicated amounts of human Aβ (pE3-42) and Aβ (1-42) peptides using β-Amyloid (pE3 Peptide) (D5N5H) Rabbit mAb (upper) and β-Amyloid (1-42 Specific) (D3E10) Rabbit mAb #12843 (lower).

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Western Blotting Image 16

Western blot analysis of brain extracts from 13-month old wild-type and TG2576 mice using β-Amyloid (1-42 Specific) (D3E10) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

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IF-F Image 17

Confocal immunofluorescent analysis of hippocampus from wild-type (left) and TG2576 (right) mice using β-Amyloid (1-40 Specific) (D8Q7I) Rabbit mAb (green) and β3-Tubulin (TU-20) Mouse mAb #4466 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

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Western Blotting Image 18

Western blot analysis of human Aβ-37, Aβ-38, Aβ-39, Aβ-40, Aβ-42, and Aβ-43 peptides (10 ng) using β-Amyloid (1-37 Specific) (D2A6H) Rabbit mAb (upper) or β-Amyloid (D54D2) XP® Rabbit mAb #8243 (lower).

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Western Blotting Image 19

Western blot analysis of human Aβ-42 peptide (1 ng) and human cerebrospinal fluid (CSF) of an AD patient using β-Amyloid (D54D2) XP® Rabbit mAb.

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IHC-P (paraffin) Image 20

Immunohistochemical analysis of paraffin-embedded human Alzheimer's brain using β-Amyloid (pE3 Peptide) (D5N5H) Rabbit mAb.

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IF-P Image 21

Confocal immunofluorescent analysis of paraffin-embedded human Alzheimer's brain using β-Amyloid (D54D2) XP® Rabbit mAb (green) and Tau (Tau46) Mouse mAb #4019 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

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IHC-P (paraffin) Image 22

Immunohistochemical analysis of paraffin-embedded Tg2576 mouse model of Alzheimer's brain using β-Amyloid (pE3 Peptide) (D5N5H) Rabbit mAb.

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IF-F Image 23

Confocal immunofluorescent analysis of wild-type (left) and Tg2576 mouse model of Alzheimer's brain (right) using β-Amyloid (pE3 Peptide) (D5N5H) Rabbit mAb (green) and β3-Tubulin (TU-20) Mouse mAb #4466 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
APP Antibody 2452 20 µl
  • WB
  • IF
H M R Mk 100 to 140 Rabbit 
β-Amyloid (1-42 Specific) (D3E10) Rabbit mAb 12843 20 µl
  • WB
H 4 Rabbit 
β-Amyloid (1-40 Specific) (D8Q7I) Rabbit mAb 12990 20 µl
  • WB
  • IF
H 4 Rabbit IgG
β-Amyloid (1-37 Specific) (D2A6H) Rabbit mAb 12467 20 µl
  • WB
H 4 Rabbit IgG
β-Amyloid (1-39 Specific) (D5Y9L) Rabbit mAb 12077 20 µl
  • WB
H 4 Rabbit IgG
β-Amyloid (D54D2) XP® Rabbit mAb 8243 20 µl
  • WB
  • IP
  • IF
H 5 Rabbit IgG
β-Amyloid (pE3 Peptide) (D5N5H) Rabbit mAb 14975 20 µl
  • WB
  • IHC
  • IF
H 4 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 

The β-Amyloid Antibody Sampler Kit provides an economical means of detecting APP and APP unmodified/modified fragments using total and fragment-specific antibodies. The kit includes enough antibody to perform two western blot experiments with each primary antibody.

Each antibody in the β-Amyloid Antibody Sampler Kit specifically detects either total APP protein or indicated modified/unmodified APP fragment. APP Antibody detects endogenous levels of several isoforms of both mature and immature amyloid beta (A4) precursor protein (APP), including APP695, APP770, and APP751. β-Amyloid (1-42 Specific) (D3E10), β-Amyloid (1-40 Specific) (D8Q7I), and β-Amyloid (1-37 Specific) (D2A6H) Rabbit mAbs recognize indicated Aβ fragments using human tissue and human APP transgenic mouse model tissue. The recommended usage of β-Amyloid (1-39 Specific) (D5Y9L) Rabbit mAb is limited to specifically detecting recombinant Aβ-39 fragments. β-Amyloid (D54D2) XP® Rabbit mAb recognizes endogenous levels of total human Aβ peptide using human tissue and human APP transgenic mouse model tissue. β-Amyloid (pE3 Peptide) (D5N5H) Rabbit mAb recognizes endogenous levels of pE3 Aβ peptide in human tissue and human APP transgenic mouse models.

APP Antibody is a polyclonal antibody produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr668 of human APP695 protein. Antibodies are purified by protein A and peptide affinity chromatography. β-Amyloid fragment-specific monoclonal antibodies are produced by immunizing rabbits with synthetic peptides corresponding to residues at the carboxy terminus of each indicated human β-Amyloid fragment. β-Amyloid (D54D2) XP® Rabbit mAb is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human β-amyloid peptide (Aβ). β-Amyloid (pE3 Peptide) (D5N5H) Rabbit mAb is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human β-amyloid (pE3) peptide.

Amyloid β (Aβ) precursor protein (APP) is a 100-140 kDa transmembrane glycoprotein that exists as several isoforms (1). The amino acid sequence of APP contains an amyloid domain, which can be processed and released by two-step proteolytic cleavage (1). The extracellular deposition and accumulation of the released Aβ fragments form the main components of amyloid plaques in Alzheimer's disease (1). Several fragments corresponding to progressive APP processing at alternative cleavage sites have been identified (2). These include Aβ (1-37), Aβ (1-39), Aβ (1-40), and Aβ (1-42) (2). These fragments can also be N-terminally modified to generate pyroglutamate-3 Aβ (pE3-peptide) (3). Fragment-specific and pan-Aβ antibodies are used to detect and examine relative levels of individual Aβ fragments.

  1. Selkoe, D.J. (1996) J Biol Chem 271, 18295-8.
  2. Selkoe, D.J. and Hardy, J. (2016) EMBO Mol Med 8, 595-608.
  3. Saido, T.C. et al. (1995) Neuron 14, 457-66.
Entrez-Gene Id
351
Swiss-Prot Acc.
P05067
For Research Use Only. Not For Use In Diagnostic Procedures.

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XP is a registered trademark of Cell Signaling Technology, Inc.

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