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PhosphoSitePlus® Resource

  • Additional protein information
  • Analytical tools

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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
APP Antibody 2452 20 µl
Western Blotting Immunofluorescence
H M R Mk 100 to 140 Rabbit 
β-Amyloid (1-42 Specific) (D3E10) Rabbit mAb 12843 20 µl
Western Blotting
H 4 Rabbit 
β-Amyloid (1-40 Specific) (D8Q7I) Rabbit mAb 12990 20 µl
Western Blotting Immunofluorescence
H 4 Rabbit IgG
β-Amyloid (1-37 Specific) (D2A6H) Rabbit mAb 12467 20 µl
Western Blotting
H 4 Rabbit IgG
β-Amyloid (1-39 Specific) (D5Y9L) Rabbit mAb 12077 20 µl
Western Blotting
H 4 Rabbit IgG
β-Amyloid (D54D2) XP® Rabbit mAb 8243 20 µl
Western Blotting Immunoprecipitation Immunofluorescence
H 5 Rabbit IgG
β-Amyloid (pE3 Peptide) (D5N5H) Rabbit mAb 14975 20 µl
Western Blotting Immunohistochemistry Immunofluorescence
H 4 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
Western Blotting
Goat 

Product Description

The β-Amyloid Antibody Sampler Kit provides an economical means of detecting APP and APP unmodified/modified fragments using total and fragment-specific antibodies. The kit includes enough antibody to perform two western blot experiments with each primary antibody.


Specificity / Sensitivity

Each antibody in the β-Amyloid Antibody Sampler Kit specifically detects either total APP protein or indicated modified/unmodified APP fragment. APP Antibody detects endogenous levels of several isoforms of both mature and immature amyloid beta (A4) precursor protein (APP), including APP695, APP770, and APP751. β-Amyloid (1-42 Specific) (D3E10), β-Amyloid (1-40 Specific) (D8Q7I), and β-Amyloid (1-37 Specific) (D2A6H) Rabbit mAbs recognize indicated Aβ fragments using human tissue and human APP transgenic mouse model tissue. The recommended usage of β-Amyloid (1-39 Specific) (D5Y9L) Rabbit mAb is limited to specifically detecting recombinant Aβ-39 fragments. β-Amyloid (D54D2) XP® Rabbit mAb recognizes endogenous levels of total human Aβ peptide using human tissue and human APP transgenic mouse model tissue. β-Amyloid (pE3 Peptide) (D5N5H) Rabbit mAb recognizes endogenous levels of pE3 Aβ peptide in human tissue and human APP transgenic mouse models.


Source / Purification

APP Antibody is a polyclonal antibody produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr668 of human APP695 protein. Antibodies are purified by protein A and peptide affinity chromatography. β-Amyloid fragment-specific monoclonal antibodies are produced by immunizing rabbits with synthetic peptides corresponding to residues at the carboxy terminus of each indicated human β-Amyloid fragment. β-Amyloid (D54D2) XP® Rabbit mAb is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human β-amyloid peptide (Aβ). β-Amyloid (pE3 Peptide) (D5N5H) Rabbit mAb is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human β-amyloid (pE3) peptide.

Amyloid β (Aβ) precursor protein (APP) is a 100-140 kDa transmembrane glycoprotein that exists as several isoforms (1). The amino acid sequence of APP contains an amyloid domain, which can be processed and released by two-step proteolytic cleavage (1). The extracellular deposition and accumulation of the released Aβ fragments form the main components of amyloid plaques in Alzheimer's disease (1). Several fragments corresponding to progressive APP processing at alternative cleavage sites have been identified (2). These include Aβ (1-37), Aβ (1-39), Aβ (1-40), and Aβ (1-42) (2). These fragments can also be N-terminally modified to generate pyroglutamate-3 Aβ (pE3-peptide) (3). Fragment-specific and pan-Aβ antibodies are used to detect and examine relative levels of individual Aβ fragments.


1.  Selkoe, D.J. (1996) J Biol Chem 271, 18295-8.

2.  Selkoe, D.J. and Hardy, J. (2016) EMBO Mol Med 8, 595-608.

3.  Saido, T.C. et al. (1995) Neuron 14, 457-66.


Entrez-Gene Id 351
Swiss-Prot Acc. P05067


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.

85314
β-Amyloid Antibody Sampler Kit