Revision 4

#8665Store at -20C

1 Kit

(7 x 20 microliters)

Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

Support: 877-678-TECH (8324)

Web: [email protected]

3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Product Includes Product # Quantity Mol. Wt Isotype/Source
LAMTOR1/C11orf59 (D11H6) XP® Rabbit mAb 8975 20 µl 18 kDa Rabbit IgG
LAMTOR2/ROBLD3 (D7C10) Rabbit mAb 8145 20 µl 14 kDa Rabbit IgG
LAMTOR3/MAPKSP1 (D38G5) Rabbit mAb 8168 20 µl 14 kDa Rabbit IgG
RagA (D8B5) Rabbit mAb 4357 20 µl 30 kDa Rabbit IgG
RagC (D8H5) Rabbit mAb 9480 20 µl 50 kDa Rabbit IgG
RagB (D18F3) Rabbit mAb 8150 20 µl 40 kDa Rabbit IgG
RagD Antibody 4470 20 µl 50 kDa Rabbit 
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl Goat 

Please visit for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.


The Rag and LAMTOR Antibody Sampler Kit is an economical means of detecting various Rag and LAMTOR proteins implicated within mTOR complex signaling. The kit contains enough primary and secondary antibody to perform two western blots with each antibody.


Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.


The mTORC kinase complex is a critical regulator of cell growth (1,2). Its activity is modulated by energy levels, growth factors, and amino acids via signaling through Akt, MAPK, and AMPK pathways (3,4). Recent studies found that the four related GTPases, RagA, RagB, RagC, and RagD, interact with raptor within the mTORC1 complex (1,2). These interactions are both necessary and sufficient for mTORC1 activation in response to amino acid signals (1,2). According to the research literature, RagD is one of several genes differentially expressed in human melanoma cell lines and has been considered to be a viable target for further diagnostic and therapeutic study (5). A protein complex consisting of LAMTOR1/C11orf59, LAMTOR2/ROBLD3, and LAMTOR3/MAPKSP1 has been identified to interact with and recruit four Rag GTPases to the surface of lysosomes (6).

  1. Sancak, Y. et al. (2008) Science 320, 1496-501.
  2. Kim, E. et al. (2008) Nat Cell Biol 10, 935-45.
  3. Hay, N. and Sonenberg, N. (2004) Genes Dev 18, 1926-45.
  4. Wullschleger, S. et al. (2006) Cell 124, 471-84.
  5. de Wit, N.J. et al. (2005) Br J Cancer 92, 2249-61.
  6. Sancak, Y. et al. (2010) Cell 141, 290-303.

Background References

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