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8665
Rag and LAMTOR Antibody Sampler Kit

Rag and LAMTOR Antibody Sampler Kit #8665

Western Blotting Image 1

Western blot analysis of extracts from various cell lines using LAMTOR1/C11orf59 (D11H6) XP® Rabbit mAb.

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Western Blotting Image 2

Western blot analysis of extracts from various cell lines using LAMTOR2/ROBLD3 (D7C10) Rabbit mAb.

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Western Blotting Image 3

Western blot analysis of extracts from various cell lines using LAMTOR3/MAPKSP1 (D38G5) Rabbit mAb.

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Western Blotting Image 4

Western blot analysis of extracts from 293 and PANC-1 cells using RagA (D8B5) Rabbit mAb.

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Western Blotting Image 5

Western blot analysis of extracts from various cell lines using RagC (D31G9) XP® Rabbit mAb.

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Western Blotting Image 6

Western blot analysis of extracts from various cell lines using RagB (D18F3) Rabbit mAb.

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Western Blotting Image 7

Western blot analysis of extracts from various cell lines using RagD Antibody.

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Western Blotting Image 8

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

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IHC-P (paraffin) Image 9

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using LAMTOR1/C11orf59 (D11H6) XP® Rabbit mAb.

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Western Blotting Image 10

Western blot analysis of extracts from mIMCD3, KNRK and COS7 cells using RagA (D8B5) Rabbit mAb.

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IHC-P (paraffin) Image 11

Immunohistochemical analysis of paraffin-embedded mouse colon using RagC (D31G9) XP® Rabbit mAb.

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IHC-P (paraffin) Image 12

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using LAMTOR1/C11orf59 (D11H6) XP® Rabbit mAb.

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IHC-P (paraffin) Image 13

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using RagC (D31G9) XP® Rabbit mAb.

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IHC-P (paraffin) Image 14

Immunohistochemical analysis of paraffin-embedded mouse colon using LAMTOR1/C11orf59 (D11H6) XP® Rabbit mAb.

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IHC-P (paraffin) Image 15

Immunohistochemical analysis of paraffin-embedded human lymphoma using RagC (D31G9) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

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IF-IC Image 16

Confocal immunofluorescent analysis of HeLa cells using LAMTOR1/C11orf59 (D11H6) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
LAMTOR1/C11orf59 (D11H6) XP® Rabbit mAb 8975 20 µl
  • WB
  • IP
  • IHC
  • IF
H M R Mk 18 Rabbit IgG
LAMTOR2/ROBLD3 (D7C10) Rabbit mAb 8145 20 µl
  • WB
  • IP
H M R Mk 14 Rabbit IgG
LAMTOR3/MAPKSP1 (D38G5) Rabbit mAb 8168 20 µl
  • WB
H M R Mk 14 Rabbit IgG
RagA (D8B5) Rabbit mAb 4357 20 µl
  • WB
  • IP
H M R Mk 30 Rabbit IgG
RagC (D31G9) XP® Rabbit mAb 5466 20 µl
  • WB
  • IP
  • IHC
H M R Mk 50 Rabbit IgG
RagB (D18F3) Rabbit mAb 8150 20 µl
  • WB
  • IP
H Mk 40 Rabbit IgG
RagD Antibody 4470 20 µl
  • WB
H 50 Rabbit 
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 

The Rag and LAMTOR Antibody Sampler Kit is an economical means of detecting various Rag and LAMTOR proteins implicated within mTOR complex signaling. The kit contains enough primary and secondary antibody to perform two western blots with each antibody.

LAMTOR1/C11orf59 (D11H6) XP® Rabbit mAb, LAMTOR2/ROBLD3 (D7C10) Rabbit mAb, and LAMTOR3/MAPKSP1 (D38G5) Rabbit mAb recognize endogenous levels of each specific protein. In addition, each antibody may cross-react with other LAMTOR proteins. RagA (D8B5) Rabbit mAb recognizes endogenous levels of total RagA protein. This antibody may also recognize endogenous levels of total RagB protein. RagB (D18F3) Rabbit mAb recognizes endogenous levels of total RagB protein. RagC (D31G9) XP® Rabbit mAb recognizes endogenous levels of total RagC protein. RagD Antibody recognizes endogenous levels of total RagD protein.

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala71 of human LAMTOR1/C11orf59 protein, residues surrounding Leu31 of human LAMTOR2/ROBLD3 protein, residues surrounding Val16 of human LAMTOR3/MAPKSP1 protein, a synthetic peptide corresponding to the sequence of human RagA protein or residues in the amino terminus of human RagB, and RagC protein. Polyclonal antibody is produced by immunizing animal with a synthetic peptide corresponding to the sequence of human RagD proteins. Polyclonal antibodies are purified by protein A and peptide affinity chromatography

The mTORC kinase complex is a critical regulator of cell growth (1,2). Its activity is modulated by energy levels, growth factors, and amino acids via signaling through Akt, MAPK, and AMPK pathways (3,4). Recent studies found that the four related GTPases, RagA, RagB, RagC, and RagD, interact with raptor within the mTORC1 complex (1,2). These interactions are both necessary and sufficient for mTORC1 activation in response to amino acid signals (1,2). According to the research literature, RagD is one of several genes differentially expressed in human melanoma cell lines and has been considered to be a viable target for further diagnostic and therapeutic study (5). A protein complex consisting of LAMTOR1/C11orf59, LAMTOR2/ROBLD3, and LAMTOR3/MAPKSP1 has been identified to interact with and recruit four Rag GTPases to the surface of lysosomes (6).

  1. Sancak, Y. et al. (2008) Science 320, 1496-501.
  2. Kim, E. et al. (2008) Nat Cell Biol 10, 935-45.
  3. Sancak, Y. et al. (2010) Cell 141, 290-303.
  4. Hay, N. and Sonenberg, N. (2004) Genes Dev 18, 1926-45.
  5. Wullschleger, S. et al. (2006) Cell 124, 471-84.
  6. de Wit, N.J. et al. (2005) Br J Cancer 92, 2249-61.
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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