| Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
|---|---|---|---|---|
| COX IV (3E11) Rabbit mAb | 4850 | 20 µl | 17 kDa | Rabbit IgG |
| Cytochrome c (136F3) Rabbit mAb | 4280 | 20 µl | 14 kDa | Rabbit IgG |
| HSP60 (D6F1) XP® Rabbit mAb | 12165 | 20 µl | 60 kDa | Rabbit IgG |
| PHB1 Antibody | 2426 | 20 µl | 29 kDa | Rabbit |
| Pyruvate Dehydrogenase (C54G1) Rabbit mAb | 3205 | 20 µl | 43 kDa | Rabbit IgG |
| SDHA (D6J9M) XP® Rabbit mAb | 11998 | 20 µl | 70 kDa | Rabbit IgG |
| SOD1 (71G8) Mouse mAb | 4266 | 20 µl | 18 kDa | Mouse IgG1 |
| VDAC (D73D12) Rabbit mAb | 4661 | 20 µl | 32 kDa | Rabbit IgG |
| Anti-mouse IgG, HRP-linked Antibody | 7076 | 100 µl | Horse | |
| Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat |
Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.
Description
The Mitochondrial Marker Antibody Sampler Kit provides an economical means to evaluate relevant mitochondial proteins. This kit contains enough primary antibody to perform two western blots per primary.
Storage
Background
The Mitochondrial Marker Antibody Sampler Kit contains a variety of antibodies directed against established mitochondrial proteins. Cytochrome c oxidase (COX) is a hetero-oligomeric enzyme consisting of 13 subunits localized to the inner mitochondrial membrane (1). Cytochrome c is a well conserved electron-transport protein and is part of the respiratory chain localized to the mitochondrial intermembrane space (2). HSP60 has primarily been known as a mitochondrial protein that is important for folding key proteins after import into the mitochondria (3). In the mitochondria, prohibitins (PHB1) mainly exist as membrane-bound ring complexes and function as chaperones maintaining mitochondrial protein stability during protein synthesis and transportation (4). In mammalian cells, the pyruvate dehydrogenase complex is located in the mitochondrial matrix (5). Succinate dehydrogenase (SDH), also known as Complex II or succinate quinone oxidoreductase, is a key component of the citric acid cycle and the electron transport chain (6). SOD1 is ubiquitously expressed and is localized in the cytosol, nucleus, and mitochondrial intermembrane space (7). Voltage-dependent anion channel (VDAC), ubiquitously expressed and located in the outer mitochondrial membrane, is generally thought to be the primary means by which metabolites diffuse in and out of the mitochondria (8).
- Ostermeier, C. et al. (1996) Curr Opin Struct Biol 6, 460-6.
- Schägger, H. (2002) Biochim Biophys Acta 1555, 154-9.
- Jindal, S. et al. (1989) Mol Cell Biol 9, 2279-83.
- Tatsuta, T. et al. (2005) Mol Biol Cell 16, 248-59.
- Strumiło, S. (2005) Acta Biochim Pol 52, 759-64.
- Oyedotun, K.S. and Lemire, B.D. (2004) J Biol Chem 279, 9424-31.
- Sherman, L. et al. (1983) Proc Natl Acad Sci U S A 80, 5465-9.
- Craigen, W.J. and Graham, B.H. (2008) J Bioenerg Biomembr 40, 207-12.
Background References
Trademarks and Patents
Limited Uses
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