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8674
Mitochondrial Marker Antibody Sampler Kit

Mitochondrial Marker Antibody Sampler Kit #8674

Western Blotting Image 1

Western blot analysis of extracts from HeLa, Jurkat and COS cell lines, using COX IV (3E11) Rabbit mAb.

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Western Blotting Image 2

Western blot analysis of cell lysates from various cell lines, using Cytochrome c (136F3) Rabbit mAb.

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Western Blotting Image 3

Western blot analysis of extracts from various cell lines using HSP60 (D6F1) XP® Rabbit mAb.

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Western Blotting Image 4

Western blot analysis of extracts from MCF-7, Neuro2A, PC12 and COS cells, using PHB1 Antibody.

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Western Blotting Image 5

Western blot analysis of extracts from various cell lines using Pyruvate Dehydrogenase (C54G1) Rabbit mAb.

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Western Blotting Image 6

Western blot analysis of extracts from various cell lines using SDHA (D6J9M) XP® Rabbit mAb.

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Western Blotting Image 7

Western blot analysis of extracts from various cell lines using SOD1 (71G8) Mouse mAb.

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Western Blotting Image 8

Western blot analysis of extracts from various cell types using VDAC (D73D12) Rabbit mAb.

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Western Blotting Image 9

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition.

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IHC-P (paraffin) Image 10

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, showing staining of the mitochondria, using COX IV (3E11) Rabbit mAb.

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IHC-P (paraffin) Image 11

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Cytochrome c (136F3) Rabbit mAb.

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IHC-P (paraffin) Image 12

Immunohistochemical analysis of paraffin-embedded mouse lung using HSP60 (D6F1) XP® Rabbit mAb.

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Western Blotting Image 13

Western blot analysis of various cell lines using Pyruvate Dehydrogenase (C54G1) Rabbit mAb.

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IP Image 14

Immunoprecipitation of SDHA from HeLa cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or SDHA (D6J9M) XP® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using SDHA (D6J9M) XP® Rabbit mAb.

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IHC-P (paraffin) Image 15

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using COX IV (3E11) Rabbit mAb in the presence of control peptide (left) or Cox IV Blocking Peptide #1034 (right).

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IHC-P (paraffin) Image 16

Immunohistochemical analysis of paraffin-embedded 4T1 syngeneic mouse tumor, using Cytochrome c (136F3) Rabbit mAb #4280.

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IHC-P (paraffin) Image 17

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using HSP60 (D6F1) XP® Rabbit mAb.

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IHC-P (paraffin) Image 18

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Pyruvate Dehydrogenase (C54G1) Rabbit mAb.

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IHC-P (paraffin) Image 19

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using SDHA (D6J9M) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

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IHC-P (paraffin) Image 20

Immunohistochemical analysis of paraffin-embedded H1650 xenograft, using COX IV Rabbit mAb. Note specific staining of human cancer cells.

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IHC-P (paraffin) Image 21

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Cytochrome c (136F3) Rabbit mAb in the presence of control peptide (left) or Cytochrome c Blocking Peptide #1033 (right).

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IHC-P (paraffin) Image 22

Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using HSP60 (D6F1) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

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IHC-P (paraffin) Image 23

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Pyruvate Dehydrogenase (C54G1) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

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IF-IC Image 24

Confocal immunofluorescent analysis of HeLa cells using SDHA (D6J9M) XP® Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

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IHC-F (frozen) Image 25

Immunohistochemical analysis of frozen H1650 xenograft, using Cox IV (3E11) Rabbit mAb.

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IHC-P (paraffin) Image 26

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma, using Cytochrome c (136F3) Rabbit mAb .

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Flow Cytometry Image 27

Flow cytometric analysis of HeLa cells using HSP60 (D6F1) XP® Rabbit mAb (blue) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

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Flow Cytometry Image 28

Flow cytometric analysis of HeLa cells, using COX IV (3E11) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).

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IF-IC Image 29

Confocal immunofluorescent analysis of A-204 cells using HSP60 (D6F1) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

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IF-IC Image 30

Confocal immunofluorescent analysis of HeLa cells labeled with COX IV (3E11) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
COX IV (3E11) Rabbit mAb 4850 20 µl
  • WB
  • IP
  • IHC
  • IF
  • F
H R Mk Z B Pg 17 Rabbit IgG
Cytochrome c (136F3) Rabbit mAb 4280 20 µl
  • WB
  • IHC
H M R Mk 14 Rabbit IgG
HSP60 (D6F1) XP® Rabbit mAb 12165 20 µl
  • WB
  • IHC
  • IF
  • F
H M R Hm Mk X Z B Pg 60 Rabbit IgG
PHB1 Antibody 2426 20 µl
  • WB
  • IP
H M R Mk 32 Rabbit 
Pyruvate Dehydrogenase (C54G1) Rabbit mAb 3205 20 µl
  • WB
  • IHC
H M R Mk 43 Rabbit IgG
SDHA (D6J9M) XP® Rabbit mAb  11998 20 µl
  • WB
  • IP
  • IHC
  • IF
H M R Hm Mk 70 Rabbit IgG
SOD1 (71G8) Mouse mAb 4266 20 µl
  • WB
  • IP
H 18 Mouse IgG1
VDAC (D73D12) Rabbit mAb 4661 20 µl
  • WB
H M R Mk 32 Rabbit IgG
Anti-mouse IgG, HRP-linked Antibody 7076 100 µl
  • WB
Horse 
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 

The Mitochondrial Marker Antibody Sampler Kit provides an economical means to evaluate relevant mitochondial proteins. This kit contains enough primary antibody to perform two western blots per primary.

Each antibody in the Mitochondrial Marker Antibody Sampler Kit recognizes endogenous levels of total respective protein. SOD1 (71G8) Mouse mAb does not cross-react with other related proteins.

Rabbit monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys29 of human COX IV protein, residues 67-78 of human cytochrome c protein, Trp68 of human HSP60 protein, the sequence of human pyruvate dehydrogenase protein, Gly166 or human SDHA protein, or residues near the amino terminus of human VDAC-1 protein. Mouse monoclonal antibody is produced by immunizing animals with full-length recombinant human SOD1 protein.

Polyclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser252 of human PHB1 protein. Polyclonal antibody is purified by protein A and peptide affinity chromatography.

The Mitochondrial Marker Antibody Sampler Kit contains a variety of antibodies directed against established mitochondrial proteins. Cytochrome c oxidase (COX) is a hetero-oligomeric enzyme consisting of 13 subunits localized to the inner mitochondrial membrane (1). Cytochrome c is a well conserved electron-transport protein and is part of the respiratory chain localized to the mitochondrial intermembrane space (2). HSP60 has primarily been known as a mitochondrial protein that is important for folding key proteins after import into the mitochondria (3). In the mitochondria, prohibitins (PHB1) mainly exist as membrane-bound ring complexes and function as chaperones maintaining mitochondrial protein stability during protein synthesis and transportation (4). In mammalian cells, the pyruvate dehydrogenase complex is located in the mitochondrial matrix (5). Succinate dehydrogenase (SDH), also known as Complex II or succinate quinone oxidoreductase, is a key component of the citric acid cycle and the electron transport chain (6). SOD1 is ubiquitously expressed and is localized in the cytosol, nucleus, and mitochondrial intermembrane space (7). Voltage-dependent anion channel (VDAC), ubiquitously expressed and located in the outer mitochondrial membrane, is generally thought to be the primary means by which metabolites diffuse in and out of the mitochondria (8).

  1. Schagger H.H. et al. (2002) Biochem. Biophys. Acta. 1555, 154-159.
  2. Ostermeier, C. et al. (1996) Curr. Opin. Struct. Biol. 6, 460-466.
  3. Jindal, S. et al. (1989) Mol. Cell Biol. 9, 2279-2283.
  4. Tatsuta, T. et al. (2005) Mol Biol Cell 16, 248-59.
  5. Strumiło, S. (2005) Acta Biochim Pol 52, 759-64.
  6. Oyedotun, K.S. and Lemire, B.D. (2004) J Biol Chem 279, 9424-31.
  7. Sherman, L. et al. (1983) Proc Natl Acad Sci U S A 80, 5465-9.
  8. Craigen, W.J. and Graham, B.H. (2008) J Bioenerg Biomembr 40, 207-12.
Entrez-Gene Id
1327 , 54205 , 3329 , 5160 , 5245 , 6389 , 6647 , 7416
Swiss-Prot Acc.
P13073 , P99999 , P10809 , P08559 , P35232 , P31040 , P00441 , P21796
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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