| Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
|---|---|---|---|---|
| CBP (D6C5) Rabbit mAb | 7389 | 20 µl | 300 kDa | Rabbit IgG |
| Acetyl-CBP (Lys1535)/p300 (Lys1499) Antibody | 4771 | 20 µl | 300 kDa | Rabbit |
| PCAF (C14G9) Rabbit mAb | 3378 | 20 µl | 93 kDa | Rabbit IgG |
| GCN5L2 (C26A10) Rabbit mAb | 3305 | 20 µl | 94 kDa | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat |
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Description
The Lysine Acetyltransferase Antibody Sampler Kit provides an economical means to examine several lysine acetyltrasferases, including: Acetyl-CBP, CBP, GCN5L2, and PCAF. The kit contains enough primary antibody to perform two western blots per primary.
Storage
Background
CREB-binding protein (CBP) and p300 are highly conserved and functionally related transcriptional co-activators that associate with transcriptional regulators and signaling molecules, integrating multiple signal transduction pathways with the transcriptional machinery (1,2). CBP/p300 also contain histone acetyltransferase (HAT) activity, allowing them to acetylate histones and other proteins (2). The role of acetylation of CBP/p300 is of particular interest (2,3). Acetylation of p300 at Lys1499 has been demonstrated to enhance its HAT activity and affect a wide variety of signaling events (4). p300/CBP-associated factor (PCAF), also known as lysine acetyl-transferase 2B (KAT2B) (5), and General Control of Amino Acid Synthesis Yeast Homolog Like 2 (GCN5L2) (6) are transcriptional adaptor proteins in addition to HATs. PCAF functions as the catalytic subunit of the PCAF transcriptional co-activator complex (5). GCN5L2 functions as the catalytic subunit of the STAGA and TFTC transcription coactivator complexes (6). PCAF and GCN5L2 acetylate histone H3 at Lys14 and histone H4 at Lys8, both of which contribute to gene activation by modulating chromatin structure and recruiting additional co-activator proteins that contain acetyl-lysine binding bromo-domains (7,8). PCAF also acetylates non-histone proteins including transcriptional activators (p53, E2F1, MyoD) and general transcription factors (TFIIEβ and TFIIF) (9-12). GCN5L2 also acetylates non-histone proteins such as transcription activators (TAT, c-Myb) (13,14), transcription co-activators (PGC1-α) (15), and nuclear receptors (Steroidogenic Factor 1) (16). Acetylation of these proteins regulates their nuclear localization, protein stability, DNA binding, and co-activator association (13-16).
- Goodman, R.H. and Smolik, S. (2000) Genes Dev 14, 1553-77.
- Chan, H.M. and La Thangue, N.B. (2001) J Cell Sci 114, 2363-73.
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- Schiltz, R.L. et al. (1999) J Biol Chem 274, 1189-92.
- Grant, P.A. et al. (1999) J Biol Chem 274, 5895-900.
- Bannister, A.J. and Miska, E.A. (2000) Cell Mol Life Sci 57, 1184-92.
- Liu, L. et al. (1999) Mol Cell Biol 19, 1202-9.
- Sartorelli, V. et al. (1999) Mol Cell 4, 725-34.
- Imhof, A. et al. (1997) Curr Biol 7, 689-92.
- Kiernan, R.E. et al. (1999) EMBO J 18, 6106-18.
- Tomita, A. et al. (2000) Oncogene 19, 444-51.
- Lerin, C. et al. (2006) Cell Metab 3, 429-38.
- Jacob, A.L. et al. (2001) J Biol Chem 276, 37659-64.
Background References
Trademarks and Patents
Limited Uses
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