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PDP - Template Name: Antibody Sampler Kit
PDP - Template ID: *******4a3ef3a
  1. Products /
  2. Primary Antibodies /
  3. Lysine Acetyltransferase Antibody Sampler Kit

Lysine Acetyltransferase Antibody Sampler Kit #8686

    Product Information

    Kit Usage Information

    Protocols

    Product Description

    The Lysine Acetyltransferase Antibody Sampler Kit provides an economical means to examine several lysine acetyltrasferases, including: Acetyl-CBP, CBP, GCN5L2, and PCAF. The kit contains enough primary antibody to perform two western blots per primary.

    Specificity / Sensitivity

    Each antibody in the Lysine Acetyltransferase Antibody Sampler Kit recognizes endogenous levels of respective target protein. The antibodies do not cross-react with other family members.

    Source / Purification

    Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of human CBP protein, human GCN5L2 protein, or human PCAF protein. Polyclonal antibodies are produced by immunizing animals with a synthetic acetylated peptide corresponding to residues surrounding Lys1535 of human CBP. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

    Background

    CREB-binding protein (CBP) and p300 are highly conserved and functionally related transcriptional co-activators that associate with transcriptional regulators and signaling molecules, integrating multiple signal transduction pathways with the transcriptional machinery (1,2). CBP/p300 also contain histone acetyltransferase (HAT) activity, allowing them to acetylate histones and other proteins (2). The role of acetylation of CBP/p300 is of particular interest (2,3). Acetylation of p300 at Lys1499 has been demonstrated to enhance its HAT activity and affect a wide variety of signaling events (4). p300/CBP-associated factor (PCAF), also known as lysine acetyl-transferase 2B (KAT2B) (5), and General Control of Amino Acid Synthesis Yeast Homolog Like 2 (GCN5L2) (6) are transcriptional adaptor proteins in addition to HATs. PCAF functions as the catalytic subunit of the PCAF transcriptional co-activator complex (5). GCN5L2 functions as the catalytic subunit of the STAGA and TFTC transcription coactivator complexes (6). PCAF and GCN5L2 acetylate histone H3 at Lys14 and histone H4 at Lys8, both of which contribute to gene activation by modulating chromatin structure and recruiting additional co-activator proteins that contain acetyl-lysine binding bromo-domains (7,8). PCAF also acetylates non-histone proteins including transcriptional activators (p53, E2F1, MyoD) and general transcription factors (TFIIEβ and TFIIF) (9-12). GCN5L2 also acetylates non-histone proteins such as transcription activators (TAT, c-Myb) (13,14), transcription co-activators (PGC1-α) (15), and nuclear receptors (Steroidogenic Factor 1) (16). Acetylation of these proteins regulates their nuclear localization, protein stability, DNA binding, and co-activator association (13-16).

    1. Goodman, R.H. and Smolik, S. (2000) Genes Dev 14, 1553-77.
    2. Chan, H.M. and La Thangue, N.B. (2001) J Cell Sci 114, 2363-73.
    3. Yuan, L.W. and Giordano, A. (2002) Oncogene 21, 2253-60.
    4. Thompson, P.R. et al. (2004) Nat Struct Mol Biol 11, 308-15.
    5. Nagy, Z. and Tora, L. (2007) Oncogene 26, 5341-57.
    6. Candau, R. et al. (1996) Mol Cell Biol 16, 593-602.
    7. Schiltz, R.L. et al. (1999) J Biol Chem 274, 1189-92.
    8. Grant, P.A. et al. (1999) J Biol Chem 274, 5895-900.
    9. Bannister, A.J. and Miska, E.A. (2000) Cell Mol Life Sci 57, 1184-92.
    10. Liu, L. et al. (1999) Mol Cell Biol 19, 1202-9.
    11. Sartorelli, V. et al. (1999) Mol Cell 4, 725-34.
    12. Imhof, A. et al. (1997) Curr Biol 7, 689-92.
    13. Kiernan, R.E. et al. (1999) EMBO J 18, 6106-18.
    14. Tomita, A. et al. (2000) Oncogene 19, 444-51.
    15. Lerin, C. et al. (2006) Cell Metab 3, 429-38.
    16. Jacob, A.L. et al. (2001) J Biol Chem 276, 37659-64.

    Limited Uses

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    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.
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