Revision 1
#12159
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For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
W, IF-IC
Reactivity:
H Mk
Sensitivity:
Endogenous
MW (kDa):
130
Source/Isotype:
Rabbit IgG
UniProt ID:
#Q15334
Entrez-Gene Id:
3996
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunofluorescence (Immunocytochemistry) | 1:50 |
Storage
Specificity/Sensitivity
Source / Purification
Background
(LLGL1), is a cytoskeletal protein implicated in regulating cellular organization, migration, and cell polarity (3). As in Drosophila, decreased expression of LLGL1 correlates with an increased incidence of cellular overgrowth and malignant transformation (4-6). In mammalian epithelial cells, LLGL1 redistributes from the cytoplasm to regions of cell-cell contact, allowing the establishment and maintainence of a polarized morphology (7). LLGL1 also plays a role in the formation of epithelial junctions via its direct interactions with PAR6 and aPKC, the latter of which has been shown to phosphorylate LLGL1 at Ser663, thus restricting its localization to the basolateral region of the cell (8). LLGL1 may also play an additional, unrealized role in cellular development and differentiation as indicated by the fact that Drosophila lgl has been implicated in controlling self-renewal and differentiation of progenitor cells (9). Recent studies in mice have suggested that a mammalian LLGL1 homolog that does not have tumor suppressor-like acitvity, LLGL2, is required for proper polarized invasion of trophoblasts and efficient branching morphogenesis during placental development (10).
Background References
- Agrawal, N. et al. (1995) Dev Biol 172, 218-29.
- Massimi, P. et al. (2008) Exp Cell Res 314, 3306-17.
- Strand, D. et al. (1995) Oncogene 11, 291-301.
- Schimanski, C.C. et al. (2005) Oncogene 24, 3100-9.
- Kuphal, S. et al. (2006) Oncogene 25, 103-10.
- Lu, X. et al. (2009) Clin Cancer Res 15, 3287-96.
- Müsch, A. et al. (2002) Mol Biol Cell 13, 158-68.
- Yamanaka, T. et al. (2003) Curr Biol 13, 734-43.
- Mechler, B.M. et al. (1985) EMBO J 4, 1551-7.
- Sripathy, S. et al. (2011) Mol Cell Biol 31, 2920-33.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
W: Western Blotting IF-IC: Immunofluorescence (Immunocytochemistry)
Cross-Reactivity Key
H: Human Mk: Monkey
Trademarks and Patents
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Limited Uses
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Revision 1
