Revision 1
#3867
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For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
W
Reactivity:
H Mk
Sensitivity:
Endogenous
MW (kDa):
50
Source/Isotype:
Rabbit
UniProt ID:
#O60906
Entrez-Gene Id:
6610
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity/Sensitivity
Source / Purification
Background
nSMase1 (also termed SMPD2) is a Mg++-dependent neutral SMase that is widely expressed and predominantly localized to the endoplasmic reticulum (3,4). This protein has also been shown to have lyso-platelet activating factor (PAF) phospholipase C activity (5). A second neutral SMase, nSMase2 (also termed SMPD3) is predominantly expressed in the brain (6). The activity of neutral SMases is regulated by oxidative stress, chemotherapeutic drugs, inflammatory cytokines, and apoptotic stimuli (1). Analysis of single and double knockouts of the SMPD2 and SMPD3 has revealed that loss of both genes leads to complete loss of neutral SMase activity with developmental defects observed with loss of nSMase2 (7,8).
Background References
- Marchesini, N. and Hannun, Y.A. (2004) Biochem Cell Biol 82, 27-44.
- Ruvolo, P.P. (2001) Leukemia 15, 1153-60.
- Tomiuk, S. et al. (1998) Proc Natl Acad Sci U S A 95, 3638-43.
- Tomiuk, S. et al. (2000) J Biol Chem 275, 5710-7.
- Sawai, H. et al. (1999) J Biol Chem 274, 38131-9.
- Hofmann, K. et al. (2000) Proc Natl Acad Sci U S A 97, 5895-900.
- Zumbansen, M. and Stoffel, W. (2002) Mol Cell Biol 22, 3633-8.
- Stoffel, W. et al. (2005) Proc Natl Acad Sci U S A 102, 4554-9.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
W: Western Blotting
Cross-Reactivity Key
H: Human Mk: Monkey
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