Revision 1

#60125

Store at -20C

OAS/RNase L Pathway Antibody Sampler Kit

1 Kit

(6 x 20 microliters)

CST Logo
Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Product Includes Product # Quantity Mol. Wt Isotype/Source
RNase L (D4B4J) Rabbit Monoclonal Antibody2728120 µl80 kDaRabbit IgG
OAS1 (D1W3A) Rabbit Monoclonal Antibody1449820 µl40, 44 kDaRabbit IgG
OAS2 (E2G4K) Rabbit Monoclonal Antibody2434420 µl70 kDaRabbit IgG
OAS3 (E4X7Z) Rabbit Monoclonal Antibody7490620 µl120 kDaRabbit IgG
OASL (E7W1R) Rabbit Monoclonal Antibody3684520 µl52 kDaRabbit IgG
PDE12 (E2V7J) Rabbit Monoclonal Antibody8849920 µl75, 70 kDaRabbit IgG
Anti-rabbit IgG, HRP-linked Antibody7074100 µlGoat

Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.

Description

The OAS/RNase L Pathway Antibody Sampler Kit provides an economical means of detecting proteins involved in the OAS/RNase L pathway. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Background

The 2'-5'-oligoadenylate synthetase (OAS)/RNase L pathway is a component of the antiviral innate immune system. The OAS family of proteins includes OAS1, OAS2, OAS3, and OASL in humans, all of which are induced in response to type 1 interferons (1-3). When activated by cytosolic double-stranded RNA, OAS1-3 generate 2’-5’-linked oligoadenylates that bind to and activate the ribonuclease, RNase L. Activated RNase L is poised to degrade viral and cellular RNA as part of the antiviral innate immune response (4). Unlike OAS1-3, OASL lacks intrinsic enzymatic activity, and research studies have suggested that OASL participates in the antiviral immune response independently of the canonical OAS/RNase L signaling pathway (5,6). Negative regulation of the OAS/RNase L pathway is governed, in part, by the PDE12 phosphodiesterase that degrades 2'-5'-oligoadenylates (7).

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.