Revision 1

#71746

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Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
W, IP, IF-IC

Reactivity:
H

Sensitivity:
Endogenous

MW (kDa):
42

Source/Isotype:
Rabbit IgG

UniProt ID:
#Q6ZWT7

Entrez-Gene Id:
129642

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:200
Immunofluorescence (Immunocytochemistry) 1:12800

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity/Sensitivity

MBOAT2 (F8Z4U) Rabbit mAb recognizes endogenous levels of total MBOAT2 protein. This antibody does not cross-react with MBOAT1 protein. Non-specific mitotic staining may be observed by immunofluorescence.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human MBOAT2 protein.

Background

Membrane-bound glycerophospholipid O-acyltransferase 2 (MBOAT2) is a phospholipid-modifying enzyme. It transfers an acyl group in an acyl-CoA to a lysophospholipid to produce a phospholipid (1,2). Studies show that MBOAT2 is a ferroptosis suppressor, and this function is independent of GPX4 or AIFM2/FSP1. MBOAT2 selectively catalyzes the transfer of monounsaturated fatty acids (MUFAs) to lysophosphatidylethanolamine (lyso-PE), producing more cellular PE-MUFAs and therefore reducing cellular PE-PUFAs (polyunsaturated fatty acids). MBOAT2 suppresses ferroptosis through phospholipid remodeling. In addition, MBOAT2 expression is upregulated by androgen receptor (AR) signaling in AR+ prostate cancer cells, therefore inhibiting their ferroptosis (3).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

W: Western Blotting IP: Immunoprecipitation IF-IC: Immunofluorescence (Immunocytochemistry)

Cross-Reactivity Key

H: Human

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Western blot analysis of extracts from various cell lines using MBOAT2 (F8Z4U) Rabbit mAb (upper) or GAPDH (D16H11) Rabbit mAb #5174 (lower). Negative expression of MBOAT2 protein in HuH-7 and JHH-5 cells is consistent with the predicted expression pattern.
Western Blotting Image 1: MBOAT2 (F8Z4U) Rabbit Monoclonal Antibody
Western blot analysis of extracts from various cell lines using MBOAT2 (F8Z4U) Rabbit mAb (upper) or GAPDH (D16H11) Rabbit mAb #5174 (lower). Negative expression of MBOAT2 protein in HuH-1 cells is consistent with the predicted expression pattern.
Western Blotting Image 2: MBOAT2 (F8Z4U) Rabbit Monoclonal Antibody
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human MBOAT2 protein (hMBOAT2-Myc/DDK; +), using MBOAT2 (F8Z4U) Rabbit mAb (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), or GAPDH (D16H11) Rabbit mAb #5174 (lower).
Western Blotting Image 3: MBOAT2 (F8Z4U) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Immunoprecipitation of MBOAT2 protein from PL45 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG Isotype Control #3900, and lane 3 is MBOAT2 (F8Z4U) Rabbit mAb. Western blot analysis was performed using MBOAT2 (F8Z4U) Rabbit mAb. Mouse Anti-Rabbit IgG (Light-Chain Specific) (D4W3E) mAb (HRP Conjugate) #93702 was used as a secondary antibody.
Immunoprecipitation Image 1: MBOAT2 (F8Z4U) Rabbit Monoclonal Antibody
Confocal immunofluorescent analysis of NCI-H441 cells (left, positive) and HuH-1 cells (right, negative) using MBOAT2 (F8Z4U) Rabbit mAb (green), beta-Actin (8H10D10) Mouse mAb #3700 (red), and DAPI #4083 (blue).
Immunofluorescence Image 1: MBOAT2 (F8Z4U) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.