Bisindolylmaleimide I, Hydrochloride (#9841) Datasheet with Images Cell Signaling Technology

For Research Use Only. Not for Use in Diagnostic Procedures.

Background

Bisindolylmaleimide I (BIS), also known as GF-109203X, is a potent inhibitor of PKC (1,2). In vitro, the IC₅₀ of BIS is 10-20 nM for PKCα/β/γ and 100-200 nM for PKCδ/ε isoforms. The in vitro IC₅₀for PKCζ is approximately 6 μM, indicating that BIS is a very weak inhibitor for this isoform. In in vivo cellular assays, the IC₅₀ of BIS for PKC is 0.2-2 μM (1,3).

Molecular Formula

C₂₅H₂₄N₄O₂ • HCl

Molecular Weight

448.94 g/mol

Purity

>99%

CAS

176504-36-2

Solubility

Soluble in DMSO at 1mg/ml.

Storage

Store lyophilized or in solution at -20ºC, desiccated. Protect from light. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Directions for Use

Bisindolylmaleimide I is supplied as 500 μg powder. Store at or below -20ºC. Before use, dissolve powder in 0.28 ml DMSO to make a 4 mM bisindolylmaleimide I stock solution. For working concentrations of 2 μM-4 μM, dilute DMSO stock 1:2000 to 1:1000. Treat cells with the desired concentration for 30 minutes.

Background References

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

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