FC-L
H
Endogenous
Mouse IgG1
#P15391
930
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Flow Cytometry (Live) | 1:20 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Product Description
Background
CD19 is a 95 kDa coreceptor which amplifies the signaling cascade in B cells (1). On the B cell surface, CD19 associates with CD21, CD81, and Leu-13 to exert its function. The cytoplasmic tail of CD19 has nine conserved tyrosine residues playing critical roles in CD19 mediated function by coupling signaling molecules to the receptor (1). After B cell receptor or CD19 ligation, Tyr531 and Tyr500 of CD19 are progressively phosphorylated. This phosphorylation enables the coupling of PI3 kinase and Src family tyrosine kinase to CD19 and activates the PI3K and Src signaling pathways (2,3). Coligation of B cell receptor and CD19 also promotes Tyr409 phosphorylation in CD19. The phosphorylation at these sites enables its binding to Vav and mediates elevated intracellular calcium response, as well as the JNK pathway (4,5).
The SJ25C1 antibody is widely used to identify cells of the B-lymphocyte lineage (6).
- Tedder, T.F. et al. (1997) Immunity 6, 107-18.
- Buhl, A.M. and Cambier, J.C. (1999) J Immunol 162, 4438-46.
- Fujimoto, M. et al. (2000) Immunity 13, 47-57.
- O'Rourke, L.M. et al. (1998) Immunity 8, 635-45.
- Sato, S. et al. (1997) Proc Natl Acad Sci U S A 94, 13158-62.
- Bejcek, B.E. et al. (1995) Cancer Res 55, 2346-51.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Applications Key
FC-L: Flow Cytometry (Live)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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