IF-IC
H M R Mk
Endogenous
Rabbit IgG
#P14625
7184
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Immunofluorescence (Immunocytochemistry) | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu397 of human Grp94 protein.
Product Description
Background
Secretory proteins are synthesized on polysomes and translocated into the endoplasmic reticulum (ER). Inside ER, these proteins are often modified by disulfide bond formation, amino-linked glycosylation and folding. The ER contains a pool of molecular chaperones, including Grp94, to help ensure correct protein folding. Grp94 is a glucose-regulated protein (1) with sequence homology to Hsp90 (2). In addition to its role in helping to facilitate folding of a number of secretory proteins to their correct conformation (3), studies suggest that Grp94 derived from cancer cells also induces anti-tumor immune responses in mouse tumor models (4, 5). One way in which Grp94 promotes tumor immunogenicity is its ability to bind to and present tumor-derived peptides as antigens (6). Furthermore, Grp94 has also been shown to induce maturation of dendritic cells (7). Taken together, Grp94 functions both as a tumor-specific antigen and as an activator of antigen-presenting cells to elicit an anti-cancer immune response (8).
- Lee, A.S. et al. (1981) Proc. Natl. Acad. Sci. USA 78, 4922-4925.
- Sorger, P.K. and Pelham, H.R. (1987) J. Mol. Biol. 194, 341-344.
- Argon, Y. and Simen, B.B. (1999) Semin. Cell Dev. Biol. 10, 495-505.
- Blachere, N.E. et al. (1997) J. Exp. Med. 186, 1315-1322.
- Tamura, Y. et al. (1997) Science 278, 117-120.
- Schild, H. and Rammensee, H.G. (2000) Nat. Immunol. 1, 100-101.
- Singh-Jasuja, H. et al. (2000) Eur. J. Immunol. 30, 2211-2215.
- Nicchitta, C.V. et al. (2004) Cell Stress Chaperones 9, 325-331.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Applications Key
IF-IC: Immunofluorescence (Immunocytochemistry)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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