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84438
Grp94 (D6X2Q) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate)

Grp94 (D6X2Q) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) #84438

This product is discontinued

Storage:

Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

Grp94 (D6X2Q) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) recognizes endogenous levels of total Grp94 protein.

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu397 of human Grp94 protein.

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct immunofluorescence analysis of human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated Grp94 (D6X2Q) XP® Rabbit mAb #20292.

Secretory proteins are synthesized on polysomes and translocated into the endoplasmic reticulum (ER). Inside ER, these proteins are often modified by disulfide bond formation, amino-linked glycosylation and folding. The ER contains a pool of molecular chaperones, including Grp94, to help ensure correct protein folding. Grp94 is a glucose-regulated protein (1) with sequence homology to Hsp90 (2). In addition to its role in helping to facilitate folding of a number of secretory proteins to their correct conformation (3), studies suggest that Grp94 derived from cancer cells also induces anti-tumor immune responses in mouse tumor models (4, 5). One way in which Grp94 promotes tumor immunogenicity is its ability to bind to and present tumor-derived peptides as antigens (6). Furthermore, Grp94 has also been shown to induce maturation of dendritic cells (7). Taken together, Grp94 functions both as a tumor-specific antigen and as an activator of antigen-presenting cells to elicit an anti-cancer immune response (8).

  1. Lee, A.S. et al. (1981) Proc Natl Acad Sci U S A 78, 4922-5.
  2. Sorger, P.K. and Pelham, H.R. (1987) J Mol Biol 194, 341-4.
  3. Argon, Y. and Simen, B.B. (1999) Semin. Cell Dev. Biol. 10, 495-505.
  4. Blachere, N.E. et al. (1997) J. Exp. Med. 186, 1315-1322.
  5. Tamura, Y. et al. (1997) Science 278, 117-120.
  6. Schild, H. and Rammensee, H.G. (2000) Nat. Immunol. 1, 100-101.
  7. Singh-Jasuja, H. et al. (2000) Eur. J. Immunol. 30, 2211-2215.
  8. Nicchitta, C.V. et al. (2004) Cell Stress Chaperones 9, 325-331.
Entrez-Gene Id
7184
Swiss-Prot Acc.
P14625
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
DRAQ5 is a registered trademark of Biostatus Limited.
The Alexa Fluor dye conjugates in this product are sold under license from Life Technologies Corporation, for research use only excluding use in combination with DNA microarrays and high content screening (HCS).
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

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