FC-L
M
Endogenous
Hamster (Syrian) IgG
#O88713
50928
Product Information
Product Usage Information
For optimal flow cytometry results, we recommend 0.5 μg of antibody per test.
| Application | Dilution |
|---|---|
| Flow Cytometry (Live) | 1:100 |
Storage
Specificity / Sensitivity
Species Reactivity:
Mouse
Source / Purification
This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Product Description
Background
Killer cell lectin-like receptor G1 (KLRG1), also known as mast cell function associated antigen (MAFA), is an inhibitory type II transmembrane lectin-like receptor (1,2). This glycoprotein is expressed on a subset of natural killer (NK) and T cells (3,4). KLRG1 is considered a marker of T cell senescence (5,6). Studies have shown that KLRG1 is upregulated by MHC Class I molecule expression and these interactions can be mediated by lectin-like class I-specific Ly49 inhibitory receptors (1,3). KLRG1 triggering interferes with the activating TCR pathway and inhibits cytotoxicity (2). Specifically, KLRG1 binds to cadherin family ligands (E-, N-, and R-), inhibiting NK cell cytotoxicity (7). The effect that KLRG1 has on NK cell differentiation, maturation, homeostasis, and activation, make it an important target when studying innate immune regulation and chronic viral infections (8).
The 2F1 antibody is widely used to detect KLRG1 on mouse cells (1).
- Hanke, T. et al. (1998) Eur J Immunol 28, 4409-17.
- Beyersdorf, N.B. et al. (2001) Eur J Immunol 31, 3443-52.
- Corral, L. et al. (2000) Eur J Immunol 30, 920-30.
- Blaser, C. et al. (1998) J Immunol 161, 6451-4.
- Henson, S.M. and Akbar, A.N. (2009) Age (Dordr) 31, 285-91.
- Rodriguez, I.J. et al. (2020) Front Immunol 11, 604591.
- Ito, M. et al. (2006) J Exp Med 203, 289-95.
- Wang, J.M. et al. (2013) J Virol 87, 11626-36.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Applications Key
FC-L: Flow Cytometry (Live)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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