Revision 1

#4082

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Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
IF-IC, FC-FP

Description

Hoechst 33342 (bisBenzimide H33342 trihydrochloride) is supplied as a lyophilized powder in 25 mg units. It can be used to examine cellular DNA in most fluorescent applications.

Background

Hoechst 33342 is a specific fluorescent DNA stain that binds within the minor groove of double-stranded AT-rich regions. This stain can be used on both live and fixed cells (1).

Fluorescent Properties

Free dye excitation maximum = 340 nm

Free dye emission maximum = 510 nm

DNA complex excitation maximum = 355 nm

DNA complex emission maximum = 465 nm

Molecular Formula

C27H28N6O 3(HCl) 3(H2O)

Storage

The lyophilized powder is stable for 1 year if kept below 0°C with desiccant. Aqueous solutions are stable for at least 1 month if kept in the dark between 2-8°C.

Directions for Use

This product is soluble up to 50 mg/ml in deionized water or dimethylformamide yielding a clear yellow solution. Heating and/or sonication may be required. For standard fluorescence based assays, Hoechst 33342 stock solutions should be diluted in phosphate-buffered saline or mounting medium to a final working concentration of 1 μg/ml.

Applications Key

IF-IC: Immunofluorescence (Immunocytochemistry) FC-FP: Flow Cytometry (Fixed/Permeabilized)

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.