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4082
Hoechst 33342

Hoechst 33342 #4082

APPLICATIONS

IF-IC

Immunofluorescent analysis of HeLa cells using α-Tubulin (DM1A) Mouse mAb #3873 (red) and Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb #3377 (green). Blue pseudocolor = Hoechst 33342 (fluorescent DNA dye).

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Flow Cytometry

Flow cytometric analysis of Jurkat cells using Phospho-Histone H3 (Ser10) (D7N8E) XP® Rabbit mAb #53348 and Hoeschst 33342 (DNA content). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

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Hoechst 33342 (bisBenzimide H33342 trihydrochloride) is supplied as a lyophilized powder in 25 mg units. It can be used to examine cellular DNA in most fluorescent applications.

Molecular Formula:

C27H28N6O 3(HCl) 3(H2O)

  • Free dye excitation maximum = 340 nm
  • Free dye emission maximum = 510 nm
  • DNA complex excitation maximum = 355 nm
  • DNA complex emission maximum = 465 nm

This product is soluble up to 50 mg/ml in deionized water or dimethylformamide yielding a clear yellow solution. Heating and/or sonication may be required. For standard fluorescence based assays, Hoechst 33342 stock solutions should be diluted in phosphate-buffered saline or mounting medium to a final working concentration of 1 μg/ml.

Storage:

The lyophilized powder is stable for 1 year if kept below 0°C with desiccant. Aqueous solutions are stable for at least 1 month if kept in the dark between 2-8°C.

Hoechst 33342 is a specific fluorescent DNA stain that binds within the minor groove of double-stranded AT-rich regions. This stain can be used on both live and fixed cells (1). Replicating or dividing chromosomes will not stain with Hoechst 33342 DNA dye.

  1. Portugal, J. and Waring, M.J. (1988) Biochim Biophys Acta 949, 158-68.
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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Product # Size Price
4082S
25 mg $ 60.0