HTScan® ABL1 Kinase Assay Kit (#7701) Datasheet with Images Cell Signaling Technology

For Research Use Only. Not for Use in Diagnostic Procedures.

UniProt ID:

#P00519

Entrez-Gene Id:

Product Information

Storage

Antibodies are supplied in in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. . Do not aliquot the antibodies. Peptides are supplied at 6 uM in 0.001% DMSO. Enzymes are supplied in 50 mM Tris-HCL (pH 8.0), 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathion and 20% glycerol. Store at -80°C.
Keep enzymes on ice during use.
Avoid repeated freeze-thaw cycles.

Source / Purification

The GST-Kinase fusion protein was produced using a baculovirus expression system from a construct containing a human ABL1 cDNA (Pro118-Ser553) fragment amino-terminally fused to a GST-HIS6-Thrombin cleavage site. The protein was then purified by one-step affinity purification using glutathione-agarose.
The detection antibody, Phospho-Tyrosine Monoclonal Antibody (P-Tyr-100) #9411 was corresponding to mice immunized with phospho-tyrosine-containing peptides

Product Description

The kit provides a means of performing enzymatic assays with active human ABL1 kinase. It includes active ABL1 kinase (supplied as a GST fusion protein), a biotinylated substrate peptide and a phospho-tyorsine monoclonal antibody for detection of the phosphorylated form of the substrate peptide.

Molecular Formula	Peptide Substrate, Biotin-Signal Transduction Protein (Tyr160): 1830 Daltons, GST-ABL1 Kinase domain: 74,140 Daltons

Background

The c-Abl proto-oncogene encodes a nonreceptor protein tyrosine kinase that is ubiquitously expressed and highly conserved in metazoan evolution. c-Abl protein is distributed in both the nucleus and the cytoplasm of cells. It is implicated in regulating cell proliferation, differentiation, apoptosis, cell adhesion, and stress responses (1-3). c-Abl kinase activity is increased in vivo by diverse physiological stimuli including integrin activation; PDGF stimulation; and binding to c-Jun, Nck, and RFX1 (2,4). The in vivo mechanism for regulation of c-Abl kinase activity is not completely understood. Tyr245 is located in the linker region between the SH2 and catalytic domains. This positioning is conserved among Abl family members. Phosphorylation at Tyr245 is involved in the activation of c-Abl kinase (5). In addition, phosphorylation at Tyr412, which is located in the kinase activation loop of c-Abl, is required for kinase activity (6).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

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