38
#Q96PD4, #Q16552
112744, 3605
Background
IL-17A/F is a cystine-linked heterodimer of IL-17A and IL-17F (1,2). IL-17A/F is produced by Th17 cells, and γδ T cells (1,2). IL-17A/F stimulates the production of pro-inflammatory cytokines and neutrophil chemoattractants, thereby functioning as a bridge between adaptive and innate immunity (1-3). Some research studies suggest that IL-17A/F may be involved in mucosal immunity against some bacterial infections and has a putative role in some autoimmune disorders (3,4). The receptor for IL-17A/F consists of a heterodimer of IL-17RA and IL-17RC and signaling through this receptor leads to the activation of the Erk1/2 and NF-κB pathways (1).
Endotoxin
Less than 0.01 ng endotoxin/1 μg hIL-17A/F.
Purity
>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hIL-17A/F. All lots are greater than 98% pure.
Source / Purification
Recombinant human IL-17A/F (hIL-17A/F) is a heterodimer that is composed of hIL-17A Ile20-Ala155 (Accession# NP_002181) linked to hIL-17F Arg31-Glu163 (Accession# NP_443104) via the linker GGGSGGGSGGGSGGGS. hIL-17A/F was expressed in human 293 cells at Cell Signaling Technology.
Bioactivity
The bioactivity of recombinant hIL-17 A/F was determined by its ability to induce mouse IL-6 production by 3T3 MEFs WT. The ED50 of each lot is between 50-200 ng/ml.
Background
IL-17A/F is a cystine-linked heterodimer of IL-17A and IL-17F (1,2). IL-17A/F is produced by Th17 cells, and γδ T cells (1,2). IL-17A/F stimulates the production of pro-inflammatory cytokines and neutrophil chemoattractants, thereby functioning as a bridge between adaptive and innate immunity (1-3). Some research studies suggest that IL-17A/F may be involved in mucosal immunity against some bacterial infections and has a putative role in some autoimmune disorders (3,4). The receptor for IL-17A/F consists of a heterodimer of IL-17RA and IL-17RC and signaling through this receptor leads to the activation of the Erk1/2 and NF-κB pathways (1).
Background References
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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