5
#O14944
2069
Background
Epiregulin is a member of the EGF family that is highly expressed in many cancer-specific cells, cells of the placenta and macrophages (1,2). Epiregulin induces keratinocyte and corneal epithelial cell proliferation, oocyte maturation and may function in blastocyst implantation during pregnancy (3-5). Active soluble epiregulin is produced by proteolytic processing of a transmembrane precursor. Epiregulin is the only EGF family member known to bind to both the ErbB1/HER1 and ErbB4/HER4 EGF receptors (6). Epiregulin binding induces receptor homodimerization or heterodimerization with other Erb/Her receptors. Epiregulin activates the ERK1/2, and PI3K/Akt pathways (1,3).
- Toyoda, H. et al. (1997) Biochem J 326 ( Pt 1), 69-75.
- Révillion, F. et al. (2008) Ann Oncol 19, 73-80.
- Morita, S. et al. (2007) Mol Vis 13, 2119-28.
- Shirakata, Y. et al. (2000) J Biol Chem 275, 5748-53.
- Song, H. et al. (2000) Mol Endocrinol 14, 1147-61.
- Riese, D.J. et al. (1998) J Biol Chem 273, 11288-94.
Endotoxin
Less than 0.01 ng endotoxin/1 μg hEpiregulin.
Purity
>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hEpiregulin. All lots are greater than 98% pure.
Source / Purification
Recombinant human Epiregulin (hEpiregulin) Val63-Leu108 (Accession #NP_001423) was produced in E. coli at Cell Signaling Technology.
Bioactivity
The bioactivity of recombinant hEpiregulin was determined in a MCF 10A cell proliferation assay. The ED50 of each lot is between 0.10-0.85 ng/ml.
Background
Epiregulin is a member of the EGF family that is highly expressed in many cancer-specific cells, cells of the placenta and macrophages (1,2). Epiregulin induces keratinocyte and corneal epithelial cell proliferation, oocyte maturation and may function in blastocyst implantation during pregnancy (3-5). Active soluble epiregulin is produced by proteolytic processing of a transmembrane precursor. Epiregulin is the only EGF family member known to bind to both the ErbB1/HER1 and ErbB4/HER4 EGF receptors (6). Epiregulin binding induces receptor homodimerization or heterodimerization with other Erb/Her receptors. Epiregulin activates the ERK1/2, and PI3K/Akt pathways (1,3).
Background References
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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