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Description
CST's PathScan® Phospho-Stat5 (Tyr694) Sandwich ELISA Antibody Pair is being offered as an economical alternative to our PathScan® Phospho-Stat5 (Tyr694) Sandwich ELISA Kit #7113. Capture and detection antibodies (100X stocks) and an HRP-conjugated secondary antibody (1000X stock) are supplied. Sufficient reagents are supplied for 4 x 96 well ELISAs. The Phospho-Stat5 (Tyr694) Mouse Capture Antibody is coated in PBS overnight in a 96 well microplate. After blocking, cell lysate is added followed by a Stat5 Rabbit Detection Antibody and an HRP-conjugated Anti-Rabbit IgG Antibody. HRP substrate (TMB) is added for color development. The magnitude of the absorbance for this developed color is proportional to the quantity of Stat5 phosphorylated at Tyr694.
*Antibodies in this kit are custom formulations specific to the kit.
Reagents not supplied
Background
Stat5 is activated in response to a wide variety of ligands, including IL-2, GM-CSF, growth hormone, and prolactin. Phosphorylation at Tyr694 is obligatory for Stat5 activation (1,2). This phosphorylation is mediated by Src upon erythropoietin stimulation (3). Stat5 is constitutively active in some leukemic cell types (4). Phosphorylated Stat5 is found in some endothelial cells treated with IL-3, which suggests its involvement in angiogenesis and cell motility (5). Stat5a and Stat5b are independently regulated and activated in various cell types. For instance, interferon treatment predominantly activates Stat5a in U-937 cells and Stat5b in HeLa cells (6).
Background References
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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