| Product Includes | Product # | Quantity | Color |
|---|---|---|---|
| FastScan™ ELISA Microwell Strip Plate, 96 Well | 53257 | 96 tests | |
| Androgen Receptor Rabbit Capture mAb | 78682 | 1 ea | Green (Lyophilized) |
| Androgen Receptor (AR-V7 Specific) Rabbit HRP-linked mAb | 89417 | 1 ea | Red (Lyophilized) |
| FastScan™ ELISA Capture Antibody Diluent | 16076 | 3 ml | Green |
| FastScan™ ELISA HRP Antibody Diluent | 28120 | 3 ml | |
| TMB Substrate | 7004 | 11 ml | |
| STOP Solution | 7002 | 11 ml | |
| Sealing Tape | 54503 | 1 ea | |
| ELISA Wash Buffer (20X) | 9801 | 25 ml | |
| FastScan™ ELISA Cell Extraction Buffer (5X) | 69905 | 10 ml | |
| FastScan™ ELISA Cell Extraction Enhancer Solution (50X) | 25243 | 1 ml | |
| FastScan™ ELISA Kit #93577 Positive Control Type 1 | 24938 | 1 ea |
*The microwell plate is supplied as 12 8-well modules - Each module is designed to break apart for 8 tests.
Description
The FastScan™ Androgen Receptor (AR-V7 Specific) ELISA Kit is a sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of AR-V7. To perform the assay, sample is incubated with a capture antibody conjugated with a proprietary tag and a second detection antibody linked to HRP, forming a sandwich with AR-V7 in solution. This entire complex is immobilized to the plate via an anti-tag antibody. The wells are then washed to remove unbound material. TMB is then added. The magnitude of observed signal is proportional to the quantity of AR-V7.
*Antibodies in this kit are custom formulations specific to kit.
Specificity/Sensitivity
Background
Androgen receptor (AR), a zinc finger transcription factor belonging to the nuclear receptor superfamily, is activated by phosphorylation and dimerization upon ligand binding (1). This promotes nuclear localization and binding of AR to androgen response elements in androgen target genes. Research studies have shown that AR plays a crucial role in several stages of male development and the progression of prostate cancer (2,3).
The AR3 or AR-V7 isoform, which lacks the typical ligand binding domain, is created through the alternative splicing of cryptic exons (4-5). AR-V7 is frequently expressed in castration-resistant prostate cancer (CRPC) and while dependent on the activity of the full-length androgen receptor (AR-FL), AR-V7 can activate a completely distinct transcriptional program (6-8). While enzalutamide and abiraterone have been beneficial in treating CRPC through the ligand binding domain of AR-FL, resistance in patients has been shown to be associated with AR-V7 detection in circulating tumor cells (9-12). Studies probing into mechanisms of overcoming this resistance are currently being explored and may help in stratifying patient populations for more personalized therapies (13-15).
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- Avila, D.M. et al. (2001) J. Steroid. Biochem. Mol. Biol. 76, 135-142.
- Montgomery, J.S. et al. (2001) J. Pathol. 195, 138-146.
- Hu, R. et al. (2009) Cancer Res 69, 16-22.
- Guo, Z. et al. (2009) Cancer Res 69, 2305-13.
- Watson, P.A. et al. (2010) Proc Natl Acad Sci U S A 107, 16759-65.
- Sun, S. et al. (2010) J Clin Invest 120, 2715-30.
- Hu, R. et al. (2012) Cancer Res 72, 3457-62.
- Scher, H.I. et al. (2012) N Engl J Med 367, 1187-97.
- de Bono, J.S. et al. (2011) N Engl J Med 364, 1995-2005.
- Ryan, C.J. et al. (2013) N Engl J Med 368, 138-48.
- Antonarakis, E.S. et al. (2014) N Engl J Med 371, 1028-38.
- Liu, C. et al. (2014) Clin Cancer Res 20, 3198-210.
- Sarwar, M. et al. (2016) Oncotarget 7, 63065-81.
- Ku, S.Y. et al. (2017) Science 355, 78-83.
Background References
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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