Mouse Essential T Cell Markers Flow Cytometry Panel (#47972) Datasheet Without Images Cell Signaling Technology

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:

FC-FP

UniProt ID:

#P06332, #P10144, #P22646, #Q9BZS1, #P11942, #P01731

Entrez-Gene Id:

12504, 3002, 12501, 50943, 12502, 12525

Product Information

Product Usage Information

All antibodies in this kit are compatible with the FoxP3/Transcription Factor Fixation/Permeabilization Kit #43481 and can be used in a single staining mix on fixed and permeabilized cells. Prior to fixation and antibody incubation, we recommend adding a fixable viability dye such as the Ghost Dye^™ Violet 510 Viability Dye to enable identification and exclusion of dead cells from analysis.

Gating strategy for identifying essential T cell subsets:
If a fixable viability dye was used, first gate on viable cells. Next, gate on lymphocytes based on forward scatter and side scatter. Conventional helper T cells are the CD3+CD4+ cells within the lymphocyte gate. Regulatory CD3+CD4+ T cells are positive for expression of FoxP3. Conventional cytotoxic T cells are the CD3+CD8+ cells within the lymphocyte gate. Both CD3+CD4+ and CD3+CD8+ cells can be positive for expression of the cytotoxic molecule Granzyme B.

Storage

CD3 (17A2) Rat mAb (PE Conjugate), CD4 (RM4-5) Rat mAb (violetFluor^™ 450 Conjugate), FoxP3 (3G3) Mouse mAb (APC Conjugate), and CD8α (2.43) Rat mAb (PE-Cy7^® Conjugate) are supplied in 10 mM NaH₂PO₄, 150 mM NaCl, 0.09% NaN₃, 0.1% gelatin, pH7.2. Granzyme B (D2H2F) Rabbit mAb (Alexa Fluor^® 488 Conjugate) is supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4oC. Do not aliquot the antibodies. Protect from light. Do not freeze.

All components in this kit are stable in accordance with the date printed on the outer packaging label when stored at the recommended temperature. Please refer to product labels, datasheets, or web pages for specific “Best By” dates for each individual component.

Specificity / Sensitivity

Each antibody in the Mouse Essential T Cell Markers Flow Cytometry Panel detects endogenous levels of its target protein. CD3 (17A2) Rat mAb (PE Conjugate), CD4 (RM4-5) Rat mAb (violetFluor^™ 450 Conjugate), and CD8α (2.43) Rat mAb (PE-Cy7^® Conjugate) detect epitopes within the extracellular domains. FoxP3 (3G3) Mouse mAb (APC Conjugate) and Granzyme B (D2H2F) Rabbit mAb (Alexa Fluor^® 488 Conjugate) detect epitopes within the intracellular domains.

Species Reactivity:

Mouse

Source / Purification

Monoclonal antibodies were purified from tissue culture supernatant via affinity chromatography. The purified antibodies were conjugated under optimal conditions, with unreacted dye removed from the preparation.

Product Description

The Mouse Essential T Cell Markers Flow Cytometry Panel can be used to identify major subsets of mouse T cells.

T cells are identified by expression of CD3. There are two major subsets of conventional T cells: helper T cells which express CD4, and cytotoxic T cells which express CD8. Regulatory CD4+ T cells express FoxP3. Upon antigen stimulation, T cells upregulate expression of Granzyme B, a serine protease that is secreted by these cells to mediate target cell cytotoxicity.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Applications Key

FC-FP: Flow Cytometry (Fixed/Permeabilized)

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cy and CyDye are registered trademarks of GE Healthcare.

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or [email protected].

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