|47972S||1 Kit (50 assays)||
|Product Includes||Quantity (with Count)||Reactivity||Dilution|
|CD3 (17A2) Rat mAb (PE Conjugate) 28306||1 x 100 µg||M||1:40|
|CD4 (RM4-5) Rat mAb (violetFluor™ 450 Conjugate) 92599||1 x 100 µg||M||1:160|
|FoxP3 (3G3) Mouse mAb (APC Conjugate) 41442||1 x 100 µg||M||1:160|
|CD8α (2.43) Rat mAb (PE-Cy7® Conjugate) 87922||1 x 100 µg||M||1:80|
|Granzyme B (D2H2F) Rabbit mAb (Alexa Fluor® 488 Conjugate) 33359||1 x 100 µl||H M||1:50|
This antibody has been validated for Flow Cytometry using the FoxP3/Transcription Factor Fixation/Permeabilization Kit #43481. Please refer to the protocol provided with the kit for detailed instructions.
Protocol Id: 1804
CD3 (17A2) Rat mAb (PE Conjugate), CD4 (RM4-5) Rat mAb (violetFluor™ 450 Conjugate), FoxP3 (3G3) Mouse mAb (APC Conjugate), and CD8α (2.43) Rat mAb (PE-Cy7® Conjugate) are supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2. Granzyme B (D2H2F) Rabbit mAb (Alexa Fluor® 488 Conjugate) is supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4oC. Do not aliquot the antibodies. Protect from light. Do not freeze.
All components in this kit are stable in accordance with the date printed on the outer packaging label when stored at the recommended temperature. Please refer to product labels, datasheets, or web pages for specific “Best By” dates for each individual component.
Monoclonal antibodies were purified from tissue culture supernatant via affinity chromatography. The purified antibodies were conjugated under optimal conditions, with unreacted dye removed from the preparation.
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