WB, IP
H M R
Endogenous
110
Rabbit IgG
#Q9Y210
7225
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human TRPC6 protein.
Background
Transient receptor potential (TRP) canonical 6 (TRPC6) belongs to the superfamily of TRP cation channels. The TRPC subfamily (TRPC1-7) is a group of calcium-permeable cation channels that mediates the increase in intracellular [Ca2+] following activation by G-protein-coupled receptors or receptor tyrosine kinases (1). TRPC6 is directly activated by diacylglycerol (DAG) (2). Various tissues express TRPC6, including brain, lung, kidney, ovary and small intestine. TRPC6 exerts a variety of biological functions in various tissues. In brain, TRPC6 plays important roles in synaptic plasticity, spatial cognition (3) and protects neurons from ischemic excitotoxicity (4). In kidney, TRPC6 is expressed in mesangial cells and podocytes of renal glomeruli (5) and regulates glomerular filtration (6). Mutations in TRPC6 gene cause autosomal dominant focal segmental glomerulosclerosis (7). In smooth muscle cells, TRPC6 mediates Na+ influx followed by Ca2+ entry via Na+/Ca2+ -exchanger (NCX) reversal which leads to contraction (8, 9).
- Montell, C. (2005) Sci STKE 2005, re3.
- Hofmann, T. et al. (1999) Nature 397, 259-63.
- Liu, Y. et al. (2015) Behav Brain Res 294, 186-93.
- Li, H. et al. (2012) J Neurochem 123, 1010-8.
- Sours, S. et al. (2006) Am J Physiol Renal Physiol 290, F1507-15.
- Dryer, S.E. and Reiser, J. (2010) Am J Physiol Renal Physiol 299, F689-701.
- Reiser, J. et al. (2005) Nat Genet 37, 739-44.
- Poburko, D. et al. (2007) Circ Res 101, 1030-8.
- Dietrich, A. and Gudermann, T. (2014) Handb Exp Pharmacol 222, 157-88.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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