WB, IP
H
Endogenous
163
Rabbit
#P01023
2
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro631 of human α-2-Macroglobulin protein.
Background
α-2-Macroglobulin (A2M) is a conserved tetrameric pan protease inhibitor found in many biological fluids, including plasma and cerebral spinal fluid. A2M can bind to most proteases, as well as growth factors, hormones, and cytokines. Clearance of A2M-bound proteins is mediated by low-density lipoprotein receptor-related protein 1 (LRP1/CD91) (1). Blood A2M levels decrease with age in humans, and this decrease may promote tumor development (2,3). In rodents, A2M modulates tumor cell adhesion, migration, and growth through inhibition of PI3K/Akt and SMAD pathways (3). Conversely, interaction with MMP2 may inhibit MMP2 activity in aged rat kidney, resulting in age-related accumulation of ECM and fibrosis (4). A2M may be a useful biomarker for liver fibrosis (5).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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