WB, IP
H M
Endogenous
45
Rabbit IgG
#P55263
132
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ADK protein.
Background
Adenosine kinase (ADK) is an evolutionary conserved ribokinase that functions as a key regulator of adenosine metabolism by catalyzing the phosphorylation of adenosine to produce 5-adenosine monophosphate (AMP) (reviewed in 1). Adenosine is not only a critical component of RNA, but also functions in maintaining energy homeostasis as a precursor of ATP production, second messenger signaling through the production of cAMP, maintaining S-adenosylmethionine-dependent transmethylation and adenine-containing co-enzymes NAD and FAD, and can directly signal through its cognate receptors. ADK has two isoforms with distinct expression and subcellular localization: a nuclear long form (ADK-L) and a cytoplasmic short form (ADK-S) (2). Genetic knockout of ADK in mice results in perinatal lethality, microvesicular hepatic steatosis, and stunted growth (3). ADK dysfunction is involved in several pathologies, including diabetes, epilepsy, and cancer (4).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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