WB
H M R
Endogenous
62-67
Rabbit
#Q16352
9118
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Alha-internexin protein. Antibodies are purified by peptide affinity chromatography.
Background
Alpha-internexin is a class-IV neuronal intermediate filament that is involved in morphogenesis of neurons and is localized mostly in synaptic vesicles, primarily found in the post-synaptic compartment where it modulates neurotransmission function by interacting with different neurotransmitter receptors (1,2). Alpha-internexin interacts with tubulin and actin, suggesting its role in axonal transport and stabilization of dendrites (2-4). Expression of Alpha-internexin occurs in embryonic stages in rat at E10 in the cortex, auditory ganglion, olfactory epithelial, spinal cord and brainstem, and expression also occurs in postnatal stages in the cerebellum in mice and humans (3). Alpha-internexin expression is altered in different neurological diseases and disorders; where it decreases in bipolar disorder and increases in schizophrenia and Alzheimer's disease (4-6). Some of these findings were generated by proteomic analysis from Alzheimer's disease and control brains (4). The overexpression of Alpha-internexin enhances the neurite outgrowth during neuronal growth factor induction (7), induces the activation caspase-3, which triggers apoptosis and eventually neuronal death (8). Alpha-internexin could be playing a role in drug addiction, where chronic cocaine exposure decreases the levels of the protein, but not with morphine exposure, suggesting the possibility to interact with different receptors (5,9).
- Yuan, A. et al. (2015) Mol Psychiatry 20, 986-94.
- Suzuki, T. et al. (2018) J Neurochem 144, 390-407.
- Kirkcaldie, M.T.K. and Dwyer, S.T. (2017) Mol Cell Neurosci 84, 68-76.
- Zhou, J. et al. (2013) Clin Chim Acta 420, 62-8.
- Yuan, A. and Nixon, R.A. (2016) Brain Res Bull 126, 334-46.
- Yuan, A. et al. (2017) Cold Spring Harb Perspect Biol 9, pii: a018309. doi: 10.1101/cshperspect.a018309.
- Chien, C.L. et al. (2005) J Neurosci Res 80, 693-706.
- Lee, W.C. et al. (2012) PLoS One 7, e43883.
- Beitner-Johnson, D. et al. (1992) J Neurosci 12, 2165-76.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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