WB, IP
H Mk
Endogenous
205
Rabbit
#P29374
5926
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly813 of human ARID4A/RbBP1 protein. Antibodies are purified by peptide affinity chromatography.
Background
ARID4A/RbBP1 was originally identified as an RB binding protein, which could repress E2F-mediated transcription through the recruitment of the mSIN3A complex (1-3). Like other AT-rich interacting domain (ARID) containing proteins, ARID4A/RbBP1 functions as a tumor suppressor, and is frequently mutated in colorectal cancer (4-6). ARID4A/RbBP1 and ARID4B/RbBP1L1 are mainly expressed in Sertoli cells in the testes, where they form a complex and serve as coactivators for androgen receptors to regulate spermatogenesis (7).
- Defeo-Jones, D. et al. (1991) Nature 352, 251-4.
- Lai, A. et al. (1999) Oncogene 18, 2091-100.
- Lai, A. et al. (1999) Mol Cell Biol 19, 6632-41.
- Hurst, D.R. et al. (2008) J Biol Chem 283, 7438-44.
- Wu, M.Y. et al. (2008) J Natl Cancer Inst 100, 1247-59.
- Cajuso, T. et al. (2014) Int J Cancer 135, 611-23.
- Wu, R.C. et al. (2013) Proc Natl Acad Sci U S A 110, 4616-21.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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