WB
H M R Mk
Endogenous
250
Rabbit IgG
#Q8IXJ9
171023
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro1330 of human ASXL1 protein.
Background
Additional sex combs-like protein 1 (ASXL1) is a polycomb-associated protein that interacts with polycomb repressive complex 2 (PRC2), which contains the histone methyltransferase EZH2 and functions to mono-, di-, and tri-methylate histone H3 on lysine 27. These histone marks are associated with transcriptional repression (1). In addition, ASXL1 interacts with BRCA1-associated protein 1 (BAP1), the catalytic subunit of the polycomb repressive deubiquitinase complex (PR-DUB), which functions to de-ubiquitinate histone H2A at lysine 119 and activate transcription (2). ASXL1 functions as a transcriptional regulator of adipogenesis, acting to repress peroxisome proliferator-activated receptor gamma (PPARG) adipocyte differentiation. ASXL1 also functions as a transcriptional regulator of hematopoiesis acting as an activator of retinoic acid receptor (RAR) mediated transcriptional activation (3,4). ASXL1 is mutated or deleted in 10 to 30 percent of all myeloid malignancies, with loss-of-function mutations associated with poor prognosis in myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML). Hematopoietic-specific deletions of ASXL1 in mice result in progressive, multi-lineage cytopenias and dysplasias, leading to increased number of hematopoietic stem and progenitor cells (5).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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