WB
H
Endogenous
45
Rabbit IgG
#Q14118
1605
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro690 of human dystroglycan 1 protein.
Background
Dystroglycan 1 (DAG1) is a ubiquitously expressed glycoprotein that is post-translationally processed into two protein subunits. α-dystroglycan is secreted and localizes to the outer plasma membrane, where it binds to the transmembrane subunit, β-dystroglycan. The two proteins form a complex that connects the cytoskeleton with the extracellular matrix and plays essential roles in various cellular processes, including neuromuscular junction function and adhesion-driven cell signaling (1,2).
Phosphorylation of β-dystroglycan at Tyr892 regulates protein-protein interaction and subcellular localization (3-5). β-dystroglycan interacts with nuclear envelope proteins emerin and lamin B1 to regulate nuclear architecture and function (6).
- Bozzi, M. et al. (2009) Matrix Biol 28, 179-87.
- Moore, C.J. and Winder, S.J. (2012) Neuromuscul Disord 22, 959-65.
- James, M. et al. (2000) J Cell Sci 113 (Pt 10), 1717-26.
- Sotgia, F. et al. (2003) Biochemistry 42, 7110-23.
- Gracida-Jiménez, V. et al. (2017) Sci Rep 7, 9906.
- Vélez-Aguilera, G. et al. (2018) Biochim Biophys Acta Mol Cell Res 1865, 406-420.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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