WB, IP, ChIP
H M R Mk
Endogenous
90
Rabbit IgG
#P10244
4605
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:100 |
Chromatin IP | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human b-Myb protein.
Background
b-Myb, also known as MYBL2, is a member of the MYB transcription factor family and plays key regulatory roles in cell cycle, proliferation, and differentiation. b-Myb is essentially expressed in all proliferating cells, and b-Myb knockout mice are embryonically lethal (1,2). Importantly, b-Myb is overexpressed and reported to play a critical role in driving several cancers, including breast cancer, hepatocellular carcinoma, renal cell carcinoma, and non-small cell lung cancer (3-7).
- Ness, S.A. (2003) Blood Cells Mol Dis 31, 192-200.
- Tanaka, Y. et al. (1999) J Biol Chem 274, 28067-70.
- Bayley, R. et al. (2020) Biochim Biophys Acta Rev Cancer 1874, 188407.
- Thorner, A.R. et al. (2009) Oncogene 28, 742-51.
- Frau, M. et al. (2011) J Hepatol 55, 111-9.
- Nientiedt, M. et al. (2021) J Cancer Res Clin Oncol 147, 129-138.
- Jin, Y. et al. (2017) Int J Mol Sci 18, 860. doi: 10.3390/ijms18060860.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation ChIP: Chromatin IP
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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