WB, IP
H M R Mk
Endogenous
28
Rabbit
#P51572-2
10134
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:25 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Species predicted to react based on 100% sequence homology
The antigen sequence used to produce this antibody shares
100% sequence homology with the species listed here, but
reactivity has not been tested or confirmed to work by CST.
Use of this product with these species is not covered under
our
Product Performance Guarantee.
Bovine, Dog
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to a region surrounding Arg71 of BAP31. Antibodies were purified by peptide affinity chromatography.
Background
BAP31 (B-cell receptor-association protein 31) is a transmembrane protein associated with the endplasmic reticulum (ER) and ER-Golgi intermediates and has been implicated in protein trafficking and apoptosis (1,2). During apoptosis Bap31 is cleaved by caspase-8 at two carboxy-terminal sites which can then direct apoptotic signals between the ER and mitochondria (2-4). Association of BAP31 with the anti-apoptotic proteins Bcl-2 or Bcl-xL could function to regulate this ER-mitochondrial pathway (2,5). Several studies have shown that BAP31 can control the trafficking of select proteins between the ER and Golgi apparatus and can affect the transport of proteins to the cell surface (6-10).
- Adachi, T. et al. (1996) EMBO J 15, 1534-41.
- Ng, F.W. et al. (1997) J Cell Biol 139, 327-38.
- Breckenridge, D.G. et al. (2003) J Cell Biol 160, 1115-27.
- Chandra, D. et al. (2004) Mol Cell Biol 24, 6592-607.
- Ng, F.W. and Shore, G.C. (1998) J Biol Chem 273, 3140-3.
- Annaert, W.G. et al. (1997) J Cell Biol 139, 1397-410.
- Lambert, G. et al. (2001) J Biol Chem 276, 20340-5.
- Schamel, W.W. et al. (2003) Proc Natl Acad Sci USA 100, 9861-6.
- Paquet, M.E. et al. (2004) J Immunol 172, 7548-55.
- Szczesna-Skorupa, E. and Kemper, B. (2006) J Biol Chem 281, 4142-8.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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