WB
H
Endogenous
30
Rabbit IgG
#Q9HBU1
56033
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly245 of human BARX1 protein.
Background
BARX homeobox 1 (BARX1) is a transcription factor (TF) that was first isolated from mouse embryos and identified to function as a repressive TF by directly binding to the NCAM promoter and inhibiting its transcription (1). BARX1 also plays a key role in cellular differentiation and cytoskeletal remodeling in various tissues such as stomach, intestine, and spleen (2-4). Several studies have also established a key role for this TF in the proliferation and metastasis of many tumors. Namely, BARX1 was shown to promote the proliferation of oesophageal adenocarcinoma and renal cell carcinoma (5,6) and was identified as one of the top ten oncogenic TFs that are critical in non-small cell lung cancer (7). Moreover, silencing of BARX1 in endometrial carcinoma cells was shown to suppress ERK/MEK signaling pathway activity and reduce cell viability (8).
- Tissier-Seta, J.P. et al. (1995) Mech Dev 51, 3-15.
- Kim, B.M. et al. (2005) Dev Cell 8, 611-22.
- Makarenkova, H.P. and Meech, R. (2012) Int Rev Cell Mol Biol 297, 117-73.
- Jayewickreme, C.D. and Shivdasani, R.A. (2015) Dev Biol 405, 21-32.
- Becker, J. et al. (2015) Cancer Med 4, 1700-4.
- Sun, G. et al. (2021) Front Mol Biosci 8, 626328.
- Zhang, D.L. et al. (2018) Cancer Lett 434, 132-143.
- Lu, Y. et al. (2021) Reprod Biol 21, 100502.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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