WB, IP, ChIP
H Mk
Endogenous
210, 110
Rabbit IgG
#Q6W2J9
54880
Product Information
Product Usage Information
For optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 × 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Chromatin IP | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human BCOR protein.
Background
BCL6 corepressor protein (BCOR) is a ubiquitously expressed transcriptional repressor that is recruited to target genes by BCL6 and other transcription factors (1). BCOR interacts with and recruits histone deacetylase (HDAC) and DNA methyltransferase (DNMT) proteins to facilitate localized histone deacetylation and DNA methylation, resulting in repression of gene expression (1,2). Mutations and translocations involving BCOR have been observed in acute myeloid leukemia, bone sarcoma, and medulloblastoma, and mutations in BCOR are known to cause X-linked Oculofaciocardiodental and Lenz microphthalmia syndromes (3-6).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation ChIP: Chromatin IP
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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