Cadherin-Catenin Antibody Sampler Kit (#9961) Datasheet with Images Cell Signaling Technology

For Research Use Only. Not for Use in Diagnostic Procedures.

Product Includes	Product #	Quantity	Mol. Wt	Isotype/Source
α-E-Catenin (23B2) Rabbit mAb	3240	20 µl	100 kDa	Rabbit IgG
N-Cadherin (D4R1H) XP^® Rabbit mAb	13116	20 µl	140 kDa	Rabbit IgG
E-Cadherin (24E10) Rabbit mAb	3195	20 µl	135 kDa	Rabbit IgG
P-Cadherin (C13F9) Rabbit mAb	2189	20 µl	120 kDa	Rabbit IgG
Pan-Cadherin (28E12) Rabbit mAb	4073	20 µl	130-150 kDa	Rabbit IgG
β-Catenin (D10A8) XP^® Rabbit mAb	8480	20 µl	92 kDa	Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody	7074	100 µl		Goat

Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.

Description

This Cadherin-Catenin Antibody Sampler kit contains reagents to examine the total protein levels of key proteins found in cell-cell adherens junctions. The kit contains enough primary and secondary antibodies to perform two Western blot experiments.

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Background

Adherens junctions are dynamic structures that form cell-cell contacts and are important in development, differentiation, tissue integrity, morphology and cell polarity. They are composed of cadherins that are transmembrane proteins that bind cadherins on adjacent cells in a calcium dependent manner. On the cytoplasmic side of adherens junctions, the cadherins associate with β-catenin, γ-catenin and p120 catenin (δ). β-catenin and γ-catenin associate with α-catenin, which links the cadherin-catenin complex to the actin cytoskeleton (1,2). Recent studies indicate that cancer cells exhibit increased N-cadherin and diminished E-cadherin expression. E-cadherin is considered a suppressor of invasive cancer cell growth and this change in cadherin expression associated with cancer progression is termed the “cadherin switch”. β-catenin is one of the key downstream effectors in the Wnt signaling pathway and has been implicated in early embryonic development and tumorigenesis (3-5).

Background References

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