WB
H
Endogenous
42
Rabbit IgG
#P41597
729230
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly197 of human CCR2 protein.
Background
CCR2 is a member of the “CC-branch” of chemokine G protein-coupled receptors that regulate monocyte chemotaxis and T cell migration/activation and drive inflammation in a number of pathological conditions (1). CCR2 is the receptor for several chemokines including MCP-1, MCP-3, and MCP-4 (2-5). CCR2 transduces signals through increases in intracellular calcium levels. It has two alternative isoforms, CCR2A and CCR2B, differing in their carboxy-terminal tails with CCR2B trafficking more efficiently to the membrane (2,6). CCR2 was originally identified in the THP-1 monocyte cell line, and its expression is decreased following differentiation into macrophages (7). Knockout studies demonstrate that CCR2 is a major regulator of macrophage trafficking (8-10). In addition, research studies have shown that CCR2 functions as an alternative coreceptor with CD4 for infection of some strains of HIV (11,12).
- Zhao, Q. (2010) J Leukoc Biol 88, 41-55.
- Charo, I.F. et al. (1994) Proc Natl Acad Sci U S A 91, 2752-6.
- Franci, C. et al. (1995) J Immunol 154, 6511-7.
- Yamagami, S. et al. (1994) Biochem Biophys Res Commun 202, 1156-62.
- Combadiere, C. et al. (1995) J Biol Chem 270, 29671-5.
- Wong, L.M. et al. (1997) J Biol Chem 272, 1038-45.
- Denholm, E.M. and Stankus, G.P. (1995) Cytokine 7, 436-40.
- Kuziel, W.A. et al. (1997) Proc Natl Acad Sci U S A 94, 12053-8.
- Boring, L. et al. (1997) J Clin Invest 100, 2552-61.
- Kurihara, T. et al. (1997) J Exp Med 186, 1757-62.
- Rucker, J. et al. (1996) Cell 87, 437-46.
- Frade, J.M. et al. (1997) J Clin Invest 100, 497-502.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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