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CCR2 (D14H7) Rabbit Monoclonal Antibody #12199

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  • WB

    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 42
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    CCR2 (D14H7) Rabbit mAb recognizes endogenous levels of total CCR2 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly197 of human CCR2 protein.

    Background

    CCR2 is a member of the “CC-branch” of chemokine G protein-coupled receptors that regulate monocyte chemotaxis and T cell migration/activation and drive inflammation in a number of pathological conditions (1). CCR2 is the receptor for several chemokines including MCP-1, MCP-3, and MCP-4 (2-5). CCR2 transduces signals through increases in intracellular calcium levels. It has two alternative isoforms, CCR2A and CCR2B, differing in their carboxy-terminal tails with CCR2B trafficking more efficiently to the membrane (2,6). CCR2 was originally identified in the THP-1 monocyte cell line, and its expression is decreased following differentiation into macrophages (7). Knockout studies demonstrate that CCR2 is a major regulator of macrophage trafficking (8-10). In addition, research studies have shown that CCR2 functions as an alternative coreceptor with CD4 for infection of some strains of HIV (11,12).

    Alternate Names

    C-C chemokine receptor type 2; C-C CKR-2; C-C motif chemokine receptor 2; CC-CKR-2; CCR-2; CCR2; CCR2A; CCR2B; CD192; chemokine (C-C motif) receptor 2; CKR2; CKR2A; CKR2B; CMKBR2; FLJ78302; MCP-1 receptor; MCP-1-R; MGC103828; MGC111760; MGC168006; Monocyte chemoattractant protein 1 receptor; monocyte chemotactic protein 1 receptor

    For Research Use Only. Not for Use in Diagnostic Procedures.
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