Revision 5

#90916Store at +4C

Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

Support: 877-678-TECH (8324)

Web: [email protected] cellsignal.com

3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:

IHC-Bond, IHC-P

REACTIVITY:

H

SENSITIVITY:

Endogenous

MW (kDa):

Source/Isotype:

Mouse IgG1 kappa

UniProt ID:

#P06126

Entrez-Gene Id:

909

Product Information

Product Usage Information

Application Dilution
IHC Leica Bond 1:50
Immunohistochemistry (Paraffin) 1:50

Storage

Supplied as liquid tissue culture supernatant containing sodium azide as a preservative. Stable for 6 months when stored at 4°C. Do not aliquot the antibody.

Specificity / Sensitivity

CD1A (MTB1) Mouse mAb recognizes endogenous levels of CD1A protein. Non-specific staining was observed in brain by immunohistochemistry.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a prokaryotic recombinant fusion protein corresponding to the full external domain of human CD1A protein.

Background

CD1A is a type 1 cell surface transmembrane glycoprotein structurally similar to MHC Class 1 that is expressed in association with β2-microglobulin (1). CD1A is a member of the CD1 family and has been shown to mediate the presentation of primary lipid and glycolipid antigens to T cell lymphocytes for recognition (1,2). It is expressed by Langerhans’ cells, cortical double positive and single positive thymocytes, and by interdigitating dendritic cells (1). CD1A has been shown to identify T cell development (3).

  1. Moody, D.B. and Suliman, S. (2017) F1000Res 6, 1909.
  2. Moody, D.B. and Porcelli, S.A. (2003) Nat Rev Immunol 3, 11-22.
  3. Siddiqui, S. et al. (2015) Front Immunol 6, 337.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Applications Key

IHC-Bond: IHC Leica Bond IHC-P: Immunohistochemistry (Paraffin)

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
SignalStain is a registered trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Revision 5
#90916

CD1A (MTB1) Mouse mAb

Immunohistochemistry Image 1: CD1A (MTB1) Mouse mAb Expand Image
Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using CD1A (MTB1) Mouse mAb performed on the Leica® BOND Rx.
Immunohistochemistry Image 1: CD1A (MTB1) Mouse mAb Expand Image
Immunohistochemical analysis of paraffin-embedded normal human skin using CD1A (MTB1) Mouse mAb.
Immunohistochemistry Image 2: CD1A (MTB1) Mouse mAb Expand Image
Immunohistochemical analysis of paraffin-embedded human tonsil using CD1A (MTB1) Mouse mAb.
Immunohistochemistry Image 3: CD1A (MTB1) Mouse mAb Expand Image
Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma using CD1A (MTB1) Mouse mAb.
Immunohistochemistry Image 4: CD1A (MTB1) Mouse mAb Expand Image
Immunohistochemical analysis of paraffin-embedded human soft tissue squamous cell carcinoma using CD1A (MTB1) Mouse mAb (left) compared to concentration-matched Mouse (G3A1) mAb IgG1 Isotype Control #5415 (right).
Immunohistochemistry Image 5: CD1A (MTB1) Mouse mAb Expand Image
Immunohistochemical analysis of paraffin-embedded HPB-ALL cell pellet (left, positive) or A-673 cell pellet (right, negative) using CD1A (MTB1) Mouse mAb.