| Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
|---|---|---|---|---|
| cdc2 (POH1) Mouse mAb | 9116 | 20 µl | 34 kDa | Mouse IgG2a |
| CDK2 (78B2) Rabbit mAb | 2546 | 20 µl | 33 kDa | Rabbit |
| CDK4 (D9G3E) Rabbit mAb | 12790 | 20 µl | 30 kDa | Rabbit IgG |
| CDK6 (D4S8S) Rabbit mAb | 13331 | 20 µl | 36 kDa | Rabbit IgG |
| CDK7 (MO1) Mouse mAb | 2916 | 20 µl | 40 kDa | Mouse IgG2b |
| CDK9 (C12F7) Rabbit mAb | 2316 | 20 µl | 42, 55 kDa | Rabbit |
| Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat | |
| Anti-mouse IgG, HRP-linked Antibody | 7076 | 100 µl | Horse |
Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.
Description
The CDK Antbody Sampler Kit provides and economical means of evaluating Cdk proteins. The kit contains enough primary and secondary antibodies to perform two western blot experiments.
Storage
Background
Cyclin-dependent kinases (CDKs) are the core effectors of cell cycle progression. CDK activity is regulated through association with their cyclin partners and cyclin-dependent kinase inhibitors (CKIs) as well as by activating and inhibitory phosphorylation events. Inhibition is mediated by Wee1 and Myt1 kinases that target residues at the amino terminus of CDK1 (1,2). Dephosphorylation of these residues by cdc25 phosphatase leads to activation of CDK kinase activity (3). The CDK7/cyclinH complex is the ubiquitous mammalian CDK-activating kinase (CAK) that phosphorylates a conserved threonine residue in the T-loop domain of CDKs. The carboxy-terminal domain of RNA polymerase II is also a target of CAK as well as CDK9/cyclinT (4,5). CDK4/6 associate with cyclinD and phosphorylate retinoblastoma protein and initiate progression through the restriction point in G1 (6). CDK2 associates with cyclinE in early S phase and cyclinA later in G2. CDK1/cyclinB regulates the initiation of mitotic events (7).
- Fattaey, A. and Booher, R.N. (1997) Prog Cell Cycle Res 3, 233-40.
- Booher, R.N. et al. (1997) J Biol Chem 272, 22300-6.
- Jessus, C. and Ozon, R. (1995) Prog Cell Cycle Res 1, 215-28.
- Kaldis, P. (1999) Cell Mol Life Sci 55, 284-96.
- De Falco, G. and Giordano, A. Cancer Biol Ther 1, 342-7.
- Sherr, C.J. (1995) Trends Biochem Sci 20, 187-90.
- Morgan, D.O. (1997) Annu Rev Cell Dev Biol 13, 261-91.
Background References
Trademarks and Patents
Limited Uses
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